Imatrix 511 silk

Manufactured by Nippi

Sourced in Japan

The IMatrix-511 silk is a lab equipment product. It is a cell culture matrix that supports the attachment and growth of various cell types.

Automatically generated - may contain errors

Lab products found in correlation

Tryple select, by Thermo Fisher Scientific (5 mentions) Stemfit ak02n, by Ajinomoto (3 mentions) Penicillin, by Thermo Fisher Scientific (3 mentions) Streptomycin, by Thermo Fisher Scientific (3 mentions) Stemfit ak02n medium, by Ajinomoto (2 mentions) Glutamax, by Thermo Fisher Scientific (2 mentions) Neural basal medium, by Thermo Fisher Scientific (1 mentions) Psc neural induction medium, by Thermo Fisher Scientific (1 mentions) Nb1rgb, by Nacalai Tesque (1 mentions) Y27632 rock inhibitor, by Fujifilm (1 mentions) Penicillin streptomycin, by Nacalai Tesque (1 mentions) Nb1rgb, by RIKEN BioResource Center (1 mentions) Dulbecco modified eagle medium (dmem), by Nacalai Tesque (1 mentions) Stemfit ak02n medium, by Takara Bio (1 mentions) Recombinant human bmp4, by R&D Systems (1 mentions) A83 01, by Merck Group (1 mentions) A83 01, by Bio-Techne (1 mentions) Jak inhibitor 1, by Merck Group (1 mentions) Stemfit ak02n medium, by ReproCELL (1 mentions) Accutase, by Innovative Cell Technologies (1 mentions)

Close spelling variants of this product

Easy iMatrix-511 silk (5 citations) IMatrix-511 (103 citations) IMatrix (5 citations)

18 protocols using imatrix 511 silk

Maintenance of Peripheral Blood-Derived ciPSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

We used peripheral blood mononuclear cells-derived ciPSC lines generated at our laboratory, which have teratoma formation ability (OPUiD04-B and OPUiD05-A) [22 (link)]. The ciPSCs were maintained on iMatrix-511 silk (0.25 µg/cm², Nippi, Inc., Tokyo, Japan)-coated dishes in StemFit AK02N medium (Ajinomoto, Tokyo, Japan) at 5%
CO₂ and 37°C. They were passaged mechanically using a glass Pasteur pipet with a split ratio of 1:5 to 1:20 every 3–5 days or enzymatically using TrypLE Select (Thermo Fisher
Scientific) at a density of 1–3 × 10³/cm² every 3–5 days. The ciPSCs at passages 15–40 were used for further experiments.

KIMURA K., NAGAKURA H., TSUKAMOTO M., YOSHIDA T., SUGISAKI H., SHISHIDA K., TACHI Y., SHIMASAKI S., SUGIURA K, & HATOYA S. (2024). Canine induced pluripotent stem cells can be successfully maintained in weekend-free culture systems. The Journal of Veterinary Medical Science, 86(3), 247-257.

+ Open protocol

+ Expand

Induced Pluripotent Stem Cell Derivation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Human skin fibroblasts NB1RGB [20–22 (link)] and human iPSCs 201B7 [23 (link)] were obtained from RIKEN Bio Resource Center. NB1RGB was maintained with Dulbecco’s Modified Eagle Medium and supplemented with 10% FBS and penicillin/streptomycin (Nacalai Tesque, Japan). iPSCs C2 were derived from NB1RGB by messenger RNA (mRNA) integration-free methods using the Stemgent® StemRNATM-NM Reprogramming Kit for Reprogramming Adult and Neonatal Human Fibroblasts (Stemgent, USA) as previously reported [11 (link), 24 (link)]. iPSCs were maintained as feeder-free culture with the NutriStem™ XF/FF medium (ReproCELL, Japan) and iMatrix511 silk (Nippi, Japan). NPCs were derived from iPSCs C2 using the PSC neural induction medium (Gibco, Thermo Fisher Scientific, USA) and maintained with the neural basal medium (Gibco, Thermo Fisher Scientific) and iMatrix 511 silk according to the manufacturer’s protocols with small modifications. iPSCs and NPCs culture media were supplemented with the Y27632 ROCK inhibitor (WAKO Pure Chemical Industries, Japan) during passage; on the subsequent day, the media were replaced with fresh culture media without the ROCK inhibitor.

Shimada M., Tokumiya T., Miyake T., Tsukada K., Kanzaki N., Yanagihara H., Kobayashi J, & Matsumoto Y. (2023). Implication of E3 ligase RAD18 in UV-induced mutagenesis in human induced pluripotent stem cells and neuronal progenitor cells. Journal of Radiation Research, 64(2), 345-351.

+ Open protocol

+ Expand

ECM Substrate Coating Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

ECM substrates, including iMatrix-511 silk (Nippi, Tokyo, Japan), were diluted in Dulbecco’s modified Eagle’s medium (DMEM; Nacalai tesque, Kyoto, Japan). The substrate was placed in a dish with the opening of the resin mask facing upwards. The ECM substrate was poured and incubated at 37 °C and 5% CO₂ for 24 h (Figure 1c). The ECM was allowed to settle and coat the substrate surface while resting with the aperture facing up. After incubation, the resin mask was peeled (Figure 1d), and the substrates were washed with phosphate-buffered saline (PBS) (-) (Figure 1e).

Togo H., Terada K., Ujitsugu A., Hirose Y., Takeuchi H, & Kusunoki M. (2023). Fabrication Scaffold with High Dimensional Control for Spheroids with Undifferentiated iPS Cell Properties. Cells, 12(2), 278.

+ Open protocol

+ Expand

SIX2-GFP Reporter iPSC Generation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

We established SIX2-GFP iPSCs by transfecting the targeting vector, along with the TALEN expression plasmids (Sakuma et al., 2013 (link)), into human iPSCs (201B7) (Takahashi et al., 2007 (link)). A full description of the methods is provided in Supplemental Experimental Procedures. We obtained 23 heterozygous GFP-knockin clones among 91 clones, as determined by PCR, Southern blotting, and sequencing of the non-GFP-containing allele. Clone #12 was adapted to the feeder-free condition using StemFit AK02N medium (Takara Bio, AJ100) and plates coated with iMatrix-511 silk (Nippi, #892021), as described previously (Nakagawa et al., 2014 (link), Yoshimura et al., 2017 (link)), and further electroporated with a plasmid expressing Cre recombinase to delete the puromycin-resistant cassette. The resultant colonies were picked up in duplicate and puromycin-sensitive clones were expanded. The absence of the cassette was verified by PCR, and clones #12-8 and #12-12 were used for detailed analyses. We used clone #12-12 for most of the presented data, but consistent data were obtained for clone #12-8 (see Figure S3).

Tanigawa S., Naganuma H., Kaku Y., Era T., Sakuma T., Yamamoto T., Taguchi A, & Nishinakamura R. (2019). Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors. Stem Cell Reports, 13(2), 322-337.

+ Open protocol

+ Expand

Induction of Naïve PSCs into Mesoderm

Check if the same lab product or an alternative is used in the 5 most similar protocols

Naïve PSCs were seeded on Laminin-E8 (0.15 μg/cm 2 iMatrix 511 silk; Nippi) at 2 x 10 4 cells/cm 2 .
The Laminin-E8-coated dish was pre-incubated at 37 °C for at least 30 min. The initial induction medium was composed of Ndiff227, 2 μM A83-01 (ALK4/5/7 inhibitor; Tocris), and 2 μM PD03. The following day, the medium was changed to Ndiff277, 2 μM A83-01, 2 μM PD03, and 1 μg/ml JAK inhibitor I (Merck, Cat. 420099-1 MGCN). The medium was changed again the next day. 10 ng/ml recombinant human BMP4 (R&D systems) was optionally added for 24 hours after the induction.
Cells were dissociated with Accutase for 20-30 min.

Io S., Kabata M., Iemura Y., Semi K., Morone N., Okamoto I., Nakamura T., Kojima Y., Iwatani C., Tsuchiya H., Kaswandy B., Kondoh E., Saitou M., Yamamoto T., Mandai M., & Takashima Y. (2020). Capturing Human Trophoblast Development with Naïve Pluripotent Stem Cells In Vitro.

+ Open protocol

+ Expand

Expansion and Characterization of Gene-Edited hiPSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gene‐edited hiPSC clones were expanded and maintained the same way as parental hiPSCs, which were maintained feeder‐free on dishes coated with iMatrix‐511 silk (nippi) in StemFit AK02N (Ajinomoto, Tokyo, Japan) with 50 U of penicillin and 50 μg/mL streptomycin (Gibco) and passaged as single cells once a week at a density of 1.5 × 10³ cells/well in 6‐well plates. Karyotype analysis was performed using the Q‐banding method by Chromocenter Inc. (Tottori, Japan). Differentiation properties for the three germ layers were confirmed by teratoma formation. An amount of 1 × 10⁶ cells were injected subcutaneously into at least three NOD‐SCID mice, which were euthanized 10 to 12 weeks later to excise the tissues. After fixation in 4% paraformaldehyde (PFA) overnight at 4°C, the tissues were embedded in paraffin, sectioned, and stained with hematoxylin–eosin (HE).

Kamakura T., Jin Y., Nishio M., Nagata S., Fukuda M., Sun L., Kawai S, & Toguchida J. (2023). Collagen X Is Dispensable for Hypertrophic Differentiation and Endochondral Ossification of Human iPSC‐Derived Chondrocytes. JBMR Plus, 7(5), e10737.

+ Open protocol

+ Expand

Maintenance and Differentiation of Human iPSCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The human iPSC line Ff-I01s04 was provided by Kyoto University. Accession Number of Ff-I01s04 is ‘CVCL_C1DY’ in Cellosaurus of Expasy (Swiss Bioinfomatics Resource Portal). As we previously reported [14 (link)], all iPSC lines were maintained using the dish coated by iMatrix-511 silk (Nippi, Tokyo, Japan) and StemFit AK02N medium (ReproCELL, Yokohama, Japan). The medium was changed at day 1, 3, 5, and 6; the detachment for passage and differentiation was performed using accutase (Innovative Cell Technologies, San Diego, CA, USA) at day 7. The use of human iPSCs was approved by the ethics committee of The Institute of Medical Science, The University of Tokyo (Approval Code: 2019-4-0716).

Tsuzuki S., Yamaguchi T., Okumura T., Kasai T., Ueno Y, & Taniguchi H. (2023). PDGF Receptors and Signaling Are Required for 3D-Structure Formation and Differentiation of Human iPSC-Derived Hepatic Spheroids. International Journal of Molecular Sciences, 24(8), 7075.

+ Open protocol

+ Expand

Feeder-Free Maintenance of Human iPSCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The healthy control human iPSC line 201B7 (female white, 36 years old; Takahashi et al., 2007 (link)) was cultured in StemFit/AK02N (Ajinomoto) as feeder-free cultures. iPSCs were passaged by 0.5 × TrypLE select (Thermo Fisher Scientific) every 7 d and seeded at 1.5 × 10⁴ cells/well in six-well plate coated with 1.5 μg/ml iMatrix-511 silk (Laminin-511 E8, Nippi) in the presence of 10 μm Y27632 (Nacalai). Culture media were changed every 2 d. For on-feeder iPSC cultures, cells were maintained on mitomycin C-treated SNL murine fibroblast feeder cells in human ESC medium: DMEM/F12 (Sigma) containing 20% KnockOut serum replacement (KSR; Life Technologies), 0.1 mm nonessential amino acids (Sigma), 0.1 mm 2-mercaptoethanol (Sigma), and 4 ng/ml fibroblast growth factor 2 (FGF-2; PeproTech) in an atmosphere containing 3% CO₂.

Watanabe H., Imaizumi K., Cai T., Zhou Z., Tomita T, & Okano H. (2021). Flexible and Accurate Substrate Processing with Distinct Presenilin/γ-Secretases in Human Cortical Neurons. eNeuro, 8(2), ENEURO.0500-20.2021.

+ Open protocol

+ Expand

Xeno-free Expansion of Reprogrammed hiPSCs

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

For the present study, hiPSCs (1231A3) reprogrammed by episomal vectors (Nakagawa et al., 2014 (link)), RPCiPS771-2 reprogrammed by RNA (Stemgent), Ff-XT28s05-Abo_To reprogrammed by episomal vectors and knockout of HLA-A,B, and CIITA genes (provided by CiRA Foundation) were cultured in plastic dishes coated with iMatrix-511 silk (Nippi, 892021) fed with StemFit AK03N (Ajinomoto) under xeno-free culture conditions. Cells were dissociated into a cell suspension using Accutase^® (Sigma Aldrich, A6964) and passaged when they reached more than 70% confluence.

Al-Akashi Z., Zujur D., Kamiya D., Kato T J.r., Kondo T, & Ikeya M. (2023). Selective vulnerability of human-induced pluripotent stem cells to dihydroorotate dehydrogenase inhibition during mesenchymal stem/stromal cell purification. Frontiers in Cell and Developmental Biology, 11, 1089945.

+ Open protocol

+ Expand

Differentiation and Culturing of hiPSC-Derived Sensory Neurons

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experiments with hiPSC-derived sensory neurons were performed with Reprocell.Inc's hiPSC-derived sensory neurons (cat #RCDN004N, Reprocell.Inc). HiPSC-sensory neurons were differentiated from the StemRNA Human iPSCs line 771-3G (RCRP003N, Reprocell.Inc) and we followed the differentiation method referenced as Young et al.^{8 (link)}. The expression pattern of sensory neuron-related genes showed features similar to those in the referenced paper. One vial of frozen sensory neurons was placed in a 37 °C-water bath and warmed for about 4 min, until completely thawed. Using a P1000 pipette, the cell suspension was gently added to a 50 mL sterile conical tube. 9 mL wash medium was added dropwise into this tube. The conical tube was centrifuged at 300 × g for 5 min at room temperature. When using a 24 well plate coated with iMatrix-511 silk (Nippi), the sensory neurons were seeded at 1.5 × 10⁵ cells per well at a density of 3 × 10⁵ cells/mL. When using a CytoView MEA 24 (AXION BIOSYSTEMS) plate coated with iMatrix-511 silk, sensory neurons were centrifuged and seeded at 1.5 × 10⁵ cells/5 µL per spot at a density of 30 × 10⁶ cells/mL. They were maintained in Sensory Neuron Culture Medium (cat#RCDN103, Reprocell.Inc) at 37 °C, 5% CO₂. Half medium change was performed every 3–4 days.

Hiranuma M., Okuda Y., Fujii Y., Richard J.P, & Watanabe T. (2024). Characterization of human iPSC-derived sensory neurons and their functional assessment using multi electrode array. Scientific Reports, 14, 6011.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!