We used human apolipoprotein E4 targeted-replacement (APOE4) mice, which at the age of 16 months were switched from a normal rodent diet (ND) (Isopurina 5001; Prolab, Dewitt, NY) to a high fat, cholesterol (HFC) enriched diet (TD 88051; Harlan Teklad, Madison, WI) for 2 months as described in Malek et al., 2005 [31 (link)]. Age-matched APOE4 mice continuously maintained on a ND (APOE4-ND) were used as a control group to compare to the APOE4-HFC mice (n = 6 or 10 per group as indicated in figure legends). Eyes were prepared for immunohistochemistry or dissected and the RPE/choroid layers separated and lysed in RIPA (25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) buffer containing protease inhibitor to extract protein lysate for Western blotting. Separately, isolated retinas were also put in RNA-later for processing of RNA for quantitative Real-Time RT-PCR. All animal procedures were in agreement with the ARVO Statement for the Use of Animals in Ophthalmic and Visual Research and in compliance with Institutional Animal Care and Use Committee (IACUC) guidelines. Duke University IACUC approved this study: Protocol #A162-12-06.
Td 88051
Manufactured by Inotiv
Sourced in United States
The TD 88051 is a laboratory instrument designed for measurement and analysis. Its core function is to provide accurate and reliable data. Detailed specifications and intended use are not available.
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Lab products found in correlation
8 protocols using td 88051
1
APOE4 Mice Model for Dietary Cholesterol Study
2
Cfh Mice Aging and Diet Study
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Mice were maintained in accordance with the Institutional Animal Care and Use Committee at Duke University. Cfh+/+ (B6), Cfh−/−, and Cfh+/− mice were generated as described (36 (link)). We have confirmed that none of the mice carries the rd8 mutation (94 (link)). Aged male B6, Cfh+/− and Cfh−/− mice (n = 84; 91–110 wk) were maintained on a normal rodent chow diet (ND) (Isopurina 5001; Prolab), and a subset of cage mate and littermate mice were switched to a HFC diet (n = 53; TD 88051; Harlan Teklad) for 8 wk. All mice were housed conventionally on a middle rack under ambient light conditions to control for light exposure. Mice were randomly assigned to treatment groups with an even distribution by age.
3
High-fat Diet Effects on APP-Tg Mice
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The APP-Tg mouse [B6.Cg- Tg (APPswe, PSEN1dE9)85Dbo/J] was generated as previously described [32 (link)]. WT mice and their APP-Tg littermates were fed either a standard diet (SD) (Harlan Teklad TD.7912) or a high-fat diet (HFD) (1.0 % cholesterol, 0.5 % cholic acid, 18 % triglyceride; Harlan Teklad TD.88051, “Paigen diet”) [14 (link)] beginning at the age of 2 months. WT and APP-Tg mice were fed with SD or HFD for 2, 5 months, or 1 year. Another set of APP-Tg mice were removed from HFD after 2 months and were put back on SD for 3 months. All animal work was done in accordance with PHS guidelines and was approved by Cornell’s Institutional Animal Care and Use Committee (Protocol # 2008–0092).
4
NAFLD and A20 Tumor Induction in BALB/c Mice
5
Conditional Cox-2 Knockout Mice Model
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The procedures for all animal experiments were approved by the UCLA Animal Research Committee, and all experiments were carried out in accordance with the approved guidelines. Cox2fl/fl;LysMCre+/– (Cox2 MKO) mice, Cox2fl/fl; LysMCre–/– (FLOX), and Cox2luc/luc (Cox2 KO) mice were bred and genotyped as previously described.5 ,74 (link) C57Bl/6J (BL6) mice were from the Jackson Laboratory (Bar Harbor, ME). All mice used in experiments were approximately 4–6 months old. Equal numbers of male and female mice were used when possible. For studies involving CCHF diet (TD.88051; Envigo, Indianapolis, IN), mice were fed for either 2 weeks or 10 weeks as indicated.
6
Aging and High-Fat Diet Modulate Cfh Mouse Model
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Mice were housed and maintained in accordance with the Institutional Animal Care and Use Committee at Duke University in adherence with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research. Cfh+/− mice were generated as previously described.14 (link) We confirmed that none of the mice carried the rd8 mutation.25 (link) Aged male Cfh+/− mice (n = 67; 91–110 weeks) were maintained on a normal rodent chow diet (normal diet [ND], Isopurina 5001; Prolab, Dewitt, NY, USA), and a subset of cage- and littermate mice were switched to a HFC diet (n = 38; TD 88051; Envigo, Madison, WI, USA) for 8 weeks. Mice were randomly assigned to treatment groups with an even distribution by age.
For studies using the laser-induced CNV or the sodium iodate (NaIO3) models, male C57BL/6J aged 8 to 10 weeks were obtained from The Jackson Laboratory (Sacramento, CA, USA). A total of 6 to 12 mice were used per dosing group (n = 78 total/model). The care and use of mice for both of these studies adhered to Pfizer's Institutional Animal Care and Use Committee guidelines.
For studies using the laser-induced CNV or the sodium iodate (NaIO3) models, male C57BL/6J aged 8 to 10 weeks were obtained from The Jackson Laboratory (Sacramento, CA, USA). A total of 6 to 12 mice were used per dosing group (n = 78 total/model). The care and use of mice for both of these studies adhered to Pfizer's Institutional Animal Care and Use Committee guidelines.
7
Dietary Cholesterol and Smoke Exposure
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Groups H, HS, and HL mice were fed with a synthetic HFD (TD.88051; Envigo, Indianapolis, IN, USA) containing 15.8% fat, 1.25% cholesterol, and 0.5% sodium cholate. Group C mice were fed with standard laboratory rodent diet. After 1 month on the HFD, groups HS and HL were subjected to smoke or laser exposure while still on the HFD. Group H mice were continuously on the HFD until the experimental endpoints of groups HS and HL.
8
Dietary Impact on Aged Mice Complement Factor H
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CFH-Y/0 and CFH-H/H mice were generated and genotyped as described previously (42 (link)). No mice used in this study have the rd8 mutation (82 (link)). Old (88–98 wk) and young adult (28–32 wk) CFH-Y/0 and CFH-H/H mice were continued on a ND (Isopurina 5001; Prolab) or switched to an HFC diet (TD 88051; Envigo) for 8 wk. All mice were housed conventionally on a middle rack in the same mouse facility under ambient light conditions to control for environmental factors and microbiome fluctuations. Mice were maintained in accordance with the Institutional Animal Care and Use Committee at Duke University. The number of mice used for each experiment is provided in the figure legends.
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