Water for cell culture

Tasmanian devil cathelicidin mature peptides Saha-CATH1, 2, 3, 4, 5, 6 and 7^{44 (link)} (Supplementary Table 1) were synthesized by ChinaPeptides Co. Ltd to 95% purity by high performance liquid chromatograph (HPLC). All peptides were solubilized in water for cell culture (Sigma-Aldrich) with 0.01% glacial acetic acid at a concentration of 10 mg/mL, then serially diluted twofold in Roswell Park Memorial Institute (RPMI) 1640 media to give a final concentration of 1 mg/mL to 31.25 μg/mL.
The DFT1 cell line 1426, derived from a primary DFT1 tumour^{46 (link)}, was obtained from Menzies Institute for Medical Research, University of Tasmania. The cell line was maintained in RPMI 1640 media with 2 mM l-glutamine (Gibco) supplemented with 10% AmnioMax II (Gibco), 10% heat-inactivated foetal bovine serum (FBS) and 1% penicillin/streptomycin (Sigma-Aldrich), in T75 flasks (Nunc) at 35 °C 5% CO₂. Cells were passaged using trypsin–EDTA (Sigma-Aldrich) when 90% confluent.

2-Hydroxyethyl methacrylate (2-HEMA) was supplied by Merck (Darmstadt, Germany); ethylene glycol dimethacrylate (EGDMA), Dulbecco’s Modified Eagle’s Medium (DMEM) and dichlorodimethylsilane were from Sigma-Aldrich (Steinheim, Germany); N-(3-aminopropyl) methacrylamide hydrochloride (APMA) was from Polysciences Inc. (Warrington, PA, USA); D(+)-sucrose (99.7%) and 2,2′-azo-bis(isobutyronitrile) (AIBN) from Acros (Geel, Belgium). The Cy3 Ab Labeling Kit was supplied from Amersham/GE Healthcare (Munich, Germany). The AAV Titration ELISA was from Progen (Heidelberg, Germany) and the IGF-1 ELISA kit (Insuline like Growth Factor 1) from R&D Systems (Nordenstadt, Germany); the Beta-Glo^® Assay System was from Promega (Mannheim, Germany); the β-gal staining kit and cell proliferation reagent WST-1 were obtained from Roche Applied Science (Mannheim, Germany). Vectastain ABC HRP kit (Peroxidase, Standard) and Biotynilated Dolichos Biflorus Agglutinin (DBA) were from Vector Laboratories (Burlingame, CA, USA). The antibody anti-IGF-I (AF-291-NA) was from R&D Systems (Nordenstadt, Germany). Ultra-pure water (resistivity > 18.2 MΩ·cm) was obtained by reverse osmosis (MilliQ^®, Millipore Ibérica, Madrid, Spain) and water for cell culture was from Sigma-Aldrich (Steinheim, Germany). All other reagents were analytical grade.