2 hydroxyethyl methacrylate

Ethyl 2-bromoisobutyrate (EbiB), N,N,N′,N″,N″-pentamethy ldiethylenetriamine (PMDETA), ethyl methacrylate (EMA), 2-hydroxyethyl methacrylate (HEMA), 2-hydroxyethyl acrylate (HEA), tertiary-butyl Methacrylate (TBMA), dimethylaminoethyl methacrylate (DMAEMA), trifluoroacetic acid (TFA), and CuBr were all purchased from Macklin (Shanghai, China). The chemicals were used without further purification except for CuBr, which was purified by successively washing with glacial acetic acid and ethanol for three times, followed by drying under vacuum at 25°C for 12 h. Mucin from porcine stomach (type II) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Coumarin 6 was purchased from Aladdin Industrial Corporation (Shanghai, China). N,N-Dimethylformamide (DMF) and tetrahydrofuran (THF) were all obtained from Sinopharm Chemical Reagent Co (Shanghai, China). Near-infrared dye cypate (Ex: 780 nm, Em: 810 nm) was synthesized in our laboratory. Dulbecco’s Modified Eagle’s Medium, penicillin, streptomycin, trypsin, and fetal bovine serum were purchased from Kangyuan Corporation (Tianjin, China). 3-(4,5-Dimethylthialzol-a-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Solarbio Corp (Beijing, China). Kunming normal mice (male) were purchased from Daren Laboratory Animal Co. Ltd. (Qingdao, China).