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7 protocols using anhydrous calcium chloride cacl2

1

Preparation of Ultrapure Water

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Ultrapure water was prepared by a Milli-Q ultrapure water machine (Millipore, Boston, MA, USA). The compound 2-octanol (Standard for GC, ≥99.5%) purchased from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China) was used as an internal standard for quantitative analysis. Calcium chloride anhydrous (CaCl2) was supplied from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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2

Polyacrylamide-Alginate Hydrogel for Controlled Drug Release

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A polyacrylamide-sodium alginate dual-network hydrogel with well controlled drug-release properties was selected as the drug carrier [39 (link)]. Acrylamide (C3H5NO, Lot No. 20220401), sodium alginate ((C6H7NaO6)n, Lot No. 20210901), N, N-methylene-bis-acrylamide (C7H10N2O2, Lot No. 20210115), ammonium persulfate ((NH4)2S2O8, Lot No. 20211023) and calcium chloride anhydrous (CaCl2, Lot No. 20210710) were all purchased from Sinopharm Chemical Reagent Co., Ltd., Shanghai, China.
For its broad-spectrum antibiotic in the pharmaceutical field, the tetracycline hydrochloride (C22H24N2O8·HCl, Lot No. G2116343, Shanghai Aladdin Biochemical Technology Co., Ltd., Shanghai, China) was used as a model drug [40 (link)]. The molecular weight is 480.9 g/mol and its saturation concentration in water is 41.59 mol/m3.
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3

Multiwalled Carbon Nanotubes for Biomaterials

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Multiwalled
carbon nanotubes
(MWCNTs) that include amine-modified (NH2−) and
carboxyl-modified (COOH−) multiwalled carbon nanotubes were
provided by Chengdu Organic Chemicals Co., Ltd., Chinese Academy of
Science (Chengdu, China). Calcium chloride anhydrous (CaCl2) was purchased from Sinopharm Chemical Reagent (Shanghai, China).
Sodium alginate (Mw = 296 kDa, PDI = 1.24)
was purchased from the Hyzlin Biology Development Co., Ltd. (Qingdao,
China). All of them were used without further purification.
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4

Synthesis of Graphene Oxide Composite

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Sodium alginate (SA) and potassium dichromate (K2Cr2O7) were obtained from Tianjin Fuchen Chemical Reagent Co. Ltd., Tianjin, China. Sodium borohydride (NaBH4), diphenyl carbamide, anhydrous calcium chloride (CaCl2), and ferrous sulfate heptahydrate (FeSO4·7H2O) were bought from the Sinopharm Group Chemical Reagent Co. Ltd., Shanghai, China. Graphene oxide powder was received from Suzhou Hengqiu Technology Co. Ltd., Suzhou, China. None of the chemicals involved in the experiment were further purified. All solutions were made using deionized water.
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5

Formulation and Characterization of Lipid Nanoparticles

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Ammonium carbonate ((NH4)2CO3) and anhydrous calcium chloride (CaCl2) were purchased from Sinopharm Chemical Reagent Co, Ltd (Shanghai, China). MIT was a gift from Dalian Meilun Biotech Co, Ltd (Dalian, China). PL (S100) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-(methoxy[polyethylene glycol]-2000) (DSPE-PEG2000) were obtained from Lipoid GmbH (Ludwigshafen, German). DSPE-PEG-FA (35,000 Da) was obtained from Ponsure Biotechnology (Shanghai, China). LysoTracker Blue, Fluo-4 AM, fluorescein diacetate (FDA), propidium iodide (PI), and 1,1′-dioctadecyl-3,3,3′,3′-tetramethyl indotricarbocyanine iodide (DiR) were obtained from Thermo Fisher Scientific (Waltham, MA, USA). All other chemicals and reagents otherwise stated were from Sinopharm Chemical Reagent Co, Ltd and of analytical grade.
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6

Synthesis of Chitosan-based Hydrogels

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Anhydrous calcium chloride (CaCl2) and magnesium chloride hexahydrate (MgCl2·6H2O) were purchased from Sinopharm Chemical Reagent Co., Ltd (Beijing, People’s Republic of China). CP disodium salt tetrahydrate (C4H8N3O5PNa2·4H2O) was purchased from Sangon Biotech (Shanghai, People’s Republic of China). Chitosan (viscosity: 200–400 mPa⋅s) was purchased from Aladdin Chemistry Co., Ltd (Shanghai, People’s Republic of China). All reagents were used as received without further purification.
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7

Silk-Based Composite with MoS2 Nanoparticles

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Silkworm silk (Chinese Academy of Agricultural Sciences, Zhenjiang, China), Formic acid (FA) (Shanghai Aladdin Co., Ltd., Shanghai, China), sodium carbonate (Na2CO3) (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China), anhydrous calcium chloride (CaCl2) (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China), absolute ethanol (C2H5OH) (Sinopharm Group Chemical Reagent Co., Ltd., Beijing, China), and MoS2 nanoparticles (Jiangsu Xianfeng Nanomaterials Technology Co., Ltd., Nanjing, China).
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