Peripheral blood was lysed with ACK lysing buffer to remove erythrocytes, and 01 × 106 cells were blocked with Fc block and stained with anti-human CD3 Alexa 488 or PerCP, anti-human CD4 Alexa 647, anti-human CD8 PECy7, anti-human CD39 biotin and streptavidin APC, anti-human CD73 PE (eBioscience and Biolegend), anti-nitrotyrosine (Sigma-Aldrich), and anti-rabbit Alexa 647 or CD20 PECy7 (Biolegend). The intracellular expression of ζ-chain was analyzed with anti-human TCRζ PE antibody (Beckman Coulter). Stained cells were analyzed by flow cytometry (FACS Canto II, Becton Dickinson), with cellular debris being excluded from the analysis. Data were analyzed using the FlowJo software.
Anti human cd8 pecy7
Manufactured by Thermo Fisher Scientific
The Anti-human CD8 PECy7 is a fluorochrome-conjugated monoclonal antibody that binds to the CD8 antigen expressed on the surface of human cytotoxic T cells. It is designed for use in flow cytometry applications to identify and enumerate CD8+ T cells within a sample.
Automatically generated - may contain errors
2 protocols using anti human cd8 pecy7
1
Phenotyping Immune Cells by Flow Cytometry
2
Multiparameter Flow Cytometry Panel
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Example 3
Antibodies for cell surface staining were obtained from BD Biosciences (anti-human CD8-PE-Cy7, CD8-V500, CD45RA-APC, CD62L-PECy7), eBioscience (anti-human CD45RO-eFluor650), Immudex and Proimmune (HLA-A201-HBs183-91-PE dextramer or pentamer), and R&D Systems (human LTβ receptor-Fc chimera). Antibodies for intracellular cytokine staining were obtained from BD Biosciences (anti-human IFN-γ-APC, TNF-α-Alexa488, IL-2-PE, Granzyme-APC) and Diaclone (anti-human perforin-FITC). Intracellular cytokine staining was performed by fixing and permeabilizing cells with cytofix/cytoperm (BD Biosciences). Flow cytometry was performed using a FACS Canto flow cytometer or LSRII (BD Biosciences) and data was analyzed with FACS Diva program (BD Biosciences).
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