Imject maleimide activated klh

Manufactured by Thermo Fisher Scientific

Imject Maleimide-activated KLH is a laboratory reagent used for the conjugation of proteins to other molecules. It serves as a carrier protein for the preparation of immunogenic conjugates. The product provides a reliable and consistent method for the covalent attachment of proteins to other compounds, facilitating various immunological and biochemical applications.

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Close spelling variants of this product

KLH-Imject Maleimide-Activated mcKLH Maleimide-activated KLH Imject

2 protocols using imject maleimide activated klh

Immunogen Synthesis and Conjugation

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HGVTSAPD
(Neu5Acα2-6GalNAc) TRPAPGSTAPPA (PDT*R-STn-20-mer) was incubated
with Imject Maleimide-activated KLH (Thermo Fisher Scientific), in
accordance with the manufacturer’s instructions. The reaction
mixture was dialyzed against distilled water and then freeze-dried
(immunogen-2: Figure 1).

Naito S., Takahashi T., Onoda J., Uemura S., Ohyabu N., Takemoto H., Yamane S., Fujii I., Nishimura S.I, & Numata Y. (2017). Generation of Novel Anti-MUC1 Monoclonal Antibodies with Designed Carbohydrate Specificities Using MUC1 Glycopeptide Library. ACS Omega, 2(11), 7493-7505.

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Peptide Conjugation to Maleimide KLH

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The peptide conjugation to Maleimide Activated KLH was performed as described in the product manual of Imject Maleimide Activated KLH (Product 77605, Thermo Scientific). Briefly, 10 mg/mL solution of peptide and KLH were mixed at pH 6.5 for 2 hours at room temperature. The maleimide groups in the maleimide-activated KLH react with free sulfhydryls in the sulfhydryl-containing peptide to form stable thioether bonds of KLH-conjugated peptide.

Khurana S., Ravichandran S., Hahn M., Coyle E.M., Stonier S.W., Zak S.E., Kindrachuk J., Davey RT J.r., Dye J.M, & Chertow D.S. (2020). Longitudinal human antibody repertoire against complete viral proteome from Ebola virus survivor reveals protective sites for vaccine design. Cell host & microbe, 27(2), 262-276.e4.

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