Rosa26 tdtomato ai14

All animal experiments were authorized by the Animal Care and Use Committee of The University of Tokyo. We also complied with all relevant ethical regulations. In each experiment, we compared the genotypes of littermates maintained in a C57BL/6J background. TOPGAL, Prg4-Cre^ERT2, Rosa26-tdTomato (Ai14), and Ctnnb1-flox mice were obtained from The Jackson Laboratory (Bar Harbor, ME) [22 (link)–24 (link)]. Ctnnb1-ex3-flox mice were generated as previously described [25 (link)].

All mice were housed in the Disease Modelling Unit (DMU) of the University of East Anglia. Experimental procedures were performed in accordance with the Animal (Scientific Procedures) Act 1986 (ASPA) under the UK Home Office Project Licences 70/8824 and PP3253888. All lines were maintained on a C57BL/6J background: Pax7^{CreERT2/+41 (link)}, Talpid3^{flox/flox15 (link)}. B6.Cg-Tg(Pax7-ZsGreen)^1Kyba/J (kindly provided by M. Kyba, University of Minnesota, USA^{42 (link)}; and Rosa26-td-Tomato (Ai14, The Jackson Laboratory). Mice carrying the TA³ floxed allele were crossed with mice expressing the tamoxifen inducible cre-recombinase, CreERT2, under the control of the endogenous Pax7 promoter (Pax7^CreERT2), generating control mice and TA^{3 icSC-KO} in the same litter. Experimental mice were 8–12 weeks old, males and females were used. For genotyping DNA was extracted from ear notches and polymerase chain reaction (PCR) was performed as described^{15 (link),41 (link)}.