NSCLC cells (2 × 104 cells) suspended in serum-free DMEM were added to the upper compartment of 24-well migration chambers (Corning). Meanwhile, the lower compartment was filled with 500 µL of DMEM containing 10% FBS as the attracting agent. Cells in the upper chamber migrating through the polycarbonate membrane into the lower chamber with high nutritional content were considered to have high migration ability. Next 0.1% crystal violet was used to stain the migrated cells on the undersurface of the lower chamber at 24 h. Finally, the migrated cells were captured with a microscope.
24 well migration chamber
Manufactured by Corning
Sourced in United States
The 24-well migration chamber is a lab equipment product designed to facilitate the study of cell migration. It provides a controlled environment for observing and analyzing the movement of cells across a defined barrier or substrate. The product consists of a 24-well plate with a specialized membrane or barrier that allows for the assessment of cell migration over time.
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Lab products found in correlation
6 protocols using 24 well migration chamber
1
NSCLC Cell Migration Assay
2
Cell Migration and Invasion Assay
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Transwell chambers were used to perform a cell migration assay. Treated MKN-45 cells were resuspended at a density of 5 × 104 cells/well (200 μL) and seeded in the upper chambers (24-well migration chambers, 8.0 μm pore membrane, Corning, New York, USA) in serum-free medium. Complete medium was placed in the lower chamber. After incubation for 48 h, the nonmigrated cells in the upper chamber were removed using cotton swabs. The migrated cells on the undersurface of the chambers were fixed and stained with crystal violet. Five images of different fields were observed using a microscope, and migrated cells were measured with ImageJ software. The same transwell inserts (8 μm pores) for 24-well plates were precoated with 100 μL/insert of 0.5 mg/mL Matrigel (BD Biosciences) for 1 h at 37°C and used to assess cell invasiveness in the invasion assay.
3
Cell Migration and Invasion Assay
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Cell ability of migration and invasion were performed using 24-well migration chamber (Corning, NY) or 24-well BioCoat Matrigel Invasion Chambers (BD Biosciences, CA) with polyethylene terephthalate (PET)-based membrane of 8.0-um pore size. In Brief, 1x105 cells were suspended with serum-free culture medium and seeded in the top chambers. Complete culture medium with 10% FBS was used in the bottom chambers. The chambers were collected after 24/48 hours’ incubation. Cells which moved through the membrane were fixed with 4% PFA and stained with 0.5% crystal violet solution. The number of cells invaded through the membrane per field were counted and imaged using SPOT imaging software (Nikon) under microscope.
4
Exosome-mediated Cell Migration in Gastric Cancer
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Cell migration assay was performed using a 24-well migration chamber (Corning, NY, USA) with or without exosomes. In the exosomes group, exosomes derived from serum of GC patients were treated with neutralizing antibodies (anti-AGT, anti- SERPINH1, anti-MMP7 respectively or combined) or isotype control for 30 min, and then washed with PBS. The pretreated exosomes (30ug) and 5 × 104 SGC‐7901 cells were co-cultured in the top chamber. The bottom chamber was filled with 600 μl medium containing 20% exosomes-free FBS. After incubation for 48 h, the cells remaining at the upper surface of the membrane were removed with a cotton swab, and those that adhered to the lower surface were fixed with paraformaldehyde and stained with crystal violet. Five fields were selected randomly to count the cells invaded through the membrane and imaged under a microscope (Leica Microsystems, Wetzlar, Germany) with magnification of 100 times. Each experiment was performed three times independently and the mean number of invaded cells were used for Transwell assay assessment.
5
Cell Migration and Invasion Assay
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Cell migration and invasion assay were performed using a 24-well migration chamber (Corning, NY, USA) with or without Matrigel. Then, 5×104 cells were seeded in the top chamber with 200 μl medium containing 5% FBS. The bottom chamber was filled with 600 μl medium containing 20% FBS. After incubation for 24 h, the cells remaining at the upper surface of the membrane were removed with a cotton swab, and those that adhered to the lower surface were fixed with paraformaldehyde and stained with crystal violet. The number of cells that had invaded through the membrane per field were counted and imaged under a microscope (Leica Microsystems, Wetzlar, Germany). Each experiment was performed three times independently.
6
Cell Migration and Invasion Assays
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Cell migration and invasion assays were performed using a 24-well migration chamber (Corning, Inc.) with or without Matrigel™, respectively. For the cell migration assays, cells at a density of 5×105 in 200 µl serum-free medium were seeded onto Transwell inserts. The bottom chamber was filled with 600 µl medium containing 20% FBS. For the invasion assays, Transwell inserts were coated with 25 µg Matrigel™. After incubation for 48 h, the inserts were fixed with neat methanol at room temperature for 20 min and stained with 2% crystal violet for 30 sec at the room temperature. The number of cells which had invaded through the membrane per field was counted and imaged under a light microscope (magnification, ×200; Carl Zeiss AG).
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