For detection of chr2 abnormalities, two BAC clones were used as probes, one located distal to the BCL11A locus (2p21) as the telomeric marker and a clone from 2q11.2 as the centromeric marker. The telomeric BAC DNA (hg19 chr2:47612794 – 47782780) was labeled with a red-dUTP (AF594, Molecular Probes) by nick translation and the centromeric BAC DNA (hg19 chr2:99969552 – 100200667) was labeled with a green-dUTP (AF488, Molecular Probes). Both labeled probes were combined with sheared human DNA and hybridized in a solution containing 50 % formamide, 10 % dextran sulfate, and 2X SSC. The cells were then stained with 4,6-diamidino-2-phenylindole (DAPI) and imaged using a Nikon Eclipse 80i with a 100x/1.40 NA Plan Apo objective and Cytovision version 7.7 (Leica Biosystems).
Na plan apo objective
Manufactured by Leica
The 100x/1.40 NA Plan Apo objective is a high-quality optical lens designed for use in advanced microscopy applications. It features a numerical aperture of 1.40 and a magnification of 100x, providing excellent resolution and clarity for detailed observations. The objective is classified as a Plan Apo type, which ensures a flat, distortion-free image field.
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Lab products found in correlation
2 protocols using na plan apo objective
1
Fluorescence In Situ Hybridization for chr2 Abnormalities
2
Fluorescence In Situ Hybridization for chr2 Abnormalities
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For detection of chr2 abnormalities, two BAC clones were used as probes, one located distal to the BCL11A locus (2p21) as the telomeric marker and a clone from 2q11.2 as the centromeric marker. The telomeric BAC DNA (hg19 chr2:47612794 – 47782780) was labeled with a red-dUTP (AF594, Molecular Probes) by nick translation and the centromeric BAC DNA (hg19 chr2:99969552 – 100200667) was labeled with a green-dUTP (AF488, Molecular Probes). Both labeled probes were combined with sheared human DNA and hybridized in a solution containing 50 % formamide, 10 % dextran sulfate, and 2X SSC. The cells were then stained with 4,6-diamidino-2-phenylindole (DAPI) and imaged using a Nikon Eclipse 80i with a 100x/1.40 NA Plan Apo objective and Cytovision version 7.7 (Leica Biosystems).
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