Cells were differentiated on 6-well collagen gel plates prepared as described previously (refer “plate preparation”) and stimulated by EFS with C-Pace EP and 6-well C-Dish (IonOptics, Westwood, MA) for differentiated myotube maturation and muscle cell training under hypoxic conditions (5% O2, 5% CO2, 90% N2). The basic EFS stimulating protocol is described in Figure 2 . A movie was recorded and analyzed using an SI8000 Motion Imaging System (SONY, Tokyo, Japan) equipped with an Eclipse Ti inverted fluorescent microscope (Nikon) and a Stage Top Incubator (Tokai Hit, Shizuoka, Japan) to maintain cells under humidified conditions at 37°C and 5% CO2. Six-well hydrogel or collagen gel plates with cells were cultured in an incubator equipped with a C-Dish connected to the C-Pace EP. An EFS was applied at between 2-20V with a 2 ms interval and a 0.5-1 Hz. The movie was recorded for 270 frames at a frame rate of 27 f/s (equal to 10 s) and analyzed using associated software.
Si8000 motion imaging system
Manufactured by Sony
Sourced in Japan
The SI8000 Motion Imaging System is a specialized lab equipment designed to capture and analyze high-speed motion. It features a high-speed camera and advanced imaging software to record and process data from various experiments and applications.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using si8000 motion imaging system
1
Electrical Stimulation of Myotubes
2
Electrical Stimulation for Myotube Maturation
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The cells were differentiated on Matrigel-coated 6-well collagen gel plates and stimulated by an EFS system with C-Pace EP and 6-well C-Dish (IonOptics; Westwood, MA, USA) for differentiated myotube maturation and muscle cell training. A movie was recorded and analyzed using an SI8000 Motion Imaging System (SONY; Tokyo, Japan) equipped with an inverted fluorescent microscope (Eclipse Ti; Nikon; Tokyo, Japan) and a stage top incubator (Tokai Hit; Shizuoka, Japan) to maintain the cells under humidified conditions at 37 °C and 5% CO2. Six-well hydrogel or collagen gel plates with cells were cultured in an incubator equipped with a C-Dish connected to the C-Pace EP. An EFS was applied between 2 and 20 V with a 2-ms interval and 0.5 and 1 Hz. The movie was recorded for 270 frames at 27 f/s (equal to 10 s) and analyzed using the associated software.
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