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Mh9a4

Manufactured by BioLegend
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The MH9A4 is a laboratory equipment product from BioLegend. It serves as a core function for research applications.

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Lab products found in correlation

Uchl1, by Thermo Fisher Scientific (1 mentions) Fixation permeabilization solution kit, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Annexin 5, by BD (1 mentions) Mq1 17h12, by BD (1 mentions) Cd3 cd28 dynabead, by Thermo Fisher Scientific (1 mentions)

2 protocols using mh9a4

1

Comprehensive T cell Immunophenotyping

Cited 1 time
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Immunophenotype of T cells was performed using standard staining and flow cytometry techniques as described before41 (link),42 (link). Briefly, combinations of fluorophore-conjugated anti-human monoclonal antibodies specific for CD3 (BioLegend, OKT3, Cat#317344, 1:500), CD4 (BioLegend, OKT4, Cat#317438, 1:500), CD8 (BioLegend, SK1, Cat#344724, 1:500), CCR7 (BioLegend, G043H7, Cat#353214, 1:500), CD45RO (BioLegend, UCHL1, Cat#304244, 1:500), PD1 (eBioscience, EBIOJ105, Cat#12-2799-42, 1:500), TIM3 (BioLegend, F38-2E2, Cat#345006, 1:500), LAG3 (eBioscience, 3DS223H, Cat#15-2239-42, 1:500) were used to label T cells in flow cytometry staining buffer for 30 min on ice after Fc blocking, followed by intracellular cytokine staining with the BD Fixation/Permeabilization Solution Kit. Data were acquired on LSRFortessa (BD Biosciences) and analyzed with FlowJo software (Treestar). Cytokine antibodies included IFN-γ (eBioscience, B27, Cat#MHCIFG04, 1:500), IL9 (BioLegend, MH9A4, Cat#507614, 1:500), IL2 (BioLegend, MQ1-17H12, Cat#500342, 1:500), GrzB (BD Biosciences, GB11, Cat#561142, 1:500), or annexin V (BD Bioscience, Cat#550475, 1:50).
Liu L., Bi E., Ma X., Xiong W., Qian J., Ye L., Su P., Wang Q., Xiao L., Yang M., Lu Y, & Yi Q. (2020). Enhanced CAR-T activity against established tumors by polarizing human T cells to secrete interleukin-9. Nature Communications, 11, 5902.
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2

Naïve CD4+ T Cell Differentiation

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Naïve CD4+ T cells were isolated from peripheral blood mononuclear cells by negative selection with a kit (Miltenyi Biotec) and stimulated with human T-activator CD3/CD28 Dynabeads (Invitrogen) together with human IL-4 (10 ng/ml), human TGF-β1 (1 ng/ml), and anti– IFN-γ (10 μg/ml) for 5 days. Cells were restimulated with PMA and ionomycin for 4 hours with brefeldin A for intracellular staining of IL-9 with PE (phycoerythrin)–conjugated anti-human IL-9 antibody (MH9A4, BioLegend).
Bi E., Ma X., Lu Y., Yang M., Wang Q., Xue G., Qian J., Wang S, & Yi Q. (2017). Foxo1 and Foxp1 play opposing roles in regulating the differentiation and antitumor activity of TH9 cells programmed by IL-7. Science signaling, 10(500), eaak9741.
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