Anti caspase 9 9502

The plenti6-SIRT3 expression vector was cloned as previously reported [17 (link)]. The pCMV6-XL5-GSTP1 expression vector (SC119655) was obtained from Origene (USA). Anti-SIRT3 (#2627), Anti-PARP (#9542), Anti-GSTP1 (#3369), Anti-c-Jun (#9165), Anti-phospho-c-Jun (#2361), Anti-phospho-JNK (#9251), Anti-Bim (#2933), Anti-Bax (#5023), Anti-Caspase 9(#9502) antibodies were obtained from Cell Signaling Technology (USA). Anti-GAPDH (sc-365062), Anti-JNK (sc-571) and Anti-IgG (sc-66931) antibodies were obtained from Santa Cruz Biotechnology (USA). Doxorubicin (D1515) and cisplatin (P4394) were obtained from Sigma-Aldrich (USA), epirubicin (56390-09-1) was obtained from Cayman Chemical (USA), Sorafenib (S7397) was obtained from Selleckchem (USA). JNK inhibitor SP600125 (S5567) was obtained from Sigma-Aldrich (USA).

For Western blotting, 20 μg of protein was applied to the lanes of 4% to 12% Bis-Tris gels (Life Technologies) for electrophoresis, blotted onto Immobilon-P membranes (Millipore, Bedford, MA, USA) after electrophoresis, and incubated with the relevant primary antibody: anti-beta-actin (Chemicon, Temecula, Ca, USA); anti-PHAPI1/APRIL (ANP32B) (Abcam); anti-GFP (MBL, Nagoya, Japan); anti-Bad (9292) anti-phospho-Bad (5284), anti-Bcl-2 (2870), anti-Bak (12105), anti-Bcl-xL (2764), anti-Bcl-2 (2870), anti-phospho-Akt (4060), anti-Akt (9272), anti-caspase-3 (9662), anti-caspase-8 (9746), and anti-caspase-9 (9502) antibodies (Cell Signaling). Appropriate species-specific conjugated secondary antibody kits were commercially obtained (GE Healthcare). Proteins were detected using the ECL prime Kit (GE Healthcare) with an ImageQuant LAS4000 system (GE Healthcare).