The murine ovarian cancer cell line ID8, a gift from Dr. Katherine Roby (University of Kansas Medical Center, Kansas City, KS) [24 (link)], was cultured in DMEM supplemented with 4% fetal bovine serum (FBS) and 5 μg /ml insulin, 5 μg /ml transferrin, and 5 ng/ml sodium selenite (all Sigma-Aldrich). To generate the more aggressive Vascular Endothelial Growth Factor (VEGF)-expressing strain, we transfected ID8 tumor cells with the pUNO1 plasmid (InvivoGen) encoding murine VEGF and the blasticidin-resistance gene. To obtain stable transfectants, tumor cells were cultured in complete medium containing 10 μg/ml blasticidin (InvivoGen) for three weeks. To generated EpCAM-expressing ovarian tumor cells, ID8-VEGF cells were stably transduced with EpCAM-encoding lentviral vector. EpCAM+ tumor cells were isolated using fluorescence-activated cell sorting. RAW246.7 cells were purchased from ATCC and cultured in DMEM supplemented 10% FBS.
Raw246.7 cells
Manufactured by American Type Culture Collection
Sourced in United States
RAW246.7 cells are a mouse macrophage cell line derived from the BALB/c mouse strain. They are commonly used in immunological and cell biology research.
Automatically generated - may contain errors
Lab products found in correlation
Puno1 plasmid, by InvivoGen (2 mentions)
Insulin, by Merck Group (2 mentions)
Fetal bovine serum (fbs), by Merck Group (2 mentions)
Transferrin, by Merck Group (2 mentions)
Sodium selenite, by Merck Group (2 mentions)
Blasticidin, by InvivoGen (2 mentions)
Thp1 lucia nf κb cells, by InvivoGen (2 mentions)
Pen strep, by InvivoGen (2 mentions)
5 protocols using raw246.7 cells
1
Murine Ovarian Cancer Cell Line Development
2
Evaluating HA-NC and Nanoemulsion Cytotoxicity
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Alamar blue was used to analyze the effect of HA-NCs and its nanoemulsion on cell viability. 1 × 105 Raw 246.7 cells/well (ATCC, Manassas, VA, USA) were cultured in RPMI supplemented with 5% fetal bovine serum (FBS), 1% (v/v) MEM eagle solution, and 1% (v/v) penicillin-streptomycin at 37 °C in a humidified atmosphere containing 5% carbon dioxide. The RAW 264.7 cell line was obtained from Dr. Mario Faúndez C. Eight different concentrations (25–0.195 mg/mL) of isolated HA-NCs or NE were incubated with the cells. Triton X-100 (1:10 dilution in RPMI) was used as the positive control and untreated cells as the negative control. After 4 h of incubation, the treatments were removed and replaced with fresh RPMI. At 24 h, the medium was eliminated and Alamar Blue diluted in RPMI was added. Cell viability was quantified according to the manufacturer`s instructions.
3
Cell Culture Protocols for RAW246.7 and THP1-Lucia NF-κB Cells
4
Murine Ovarian Cancer Cell Line Development
5
Cell Culture Protocols for RAW246.7 and THP1-Lucia NF-κB Cells
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RAW246.7 cells were purchased from ATCC and cultured in DMEM supplemented 10% fetal bovine serum (FBS). THP1-Lucia™ NF-κB cells (NF-κB-inducible reporter monocytes) were purchased from InvivoGen (Cat# thp1-nfkb) and cultured in RPMI 1640, 2 mM l -glutamine, 25 mM HEPES, 10% heat-inactivated FBS, 100 μg/ml Normocin™, Pen-Strep (100 U/ml-100 μg/ml).
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