Fmoc nh peg 2 cooh 20 atoms

TentaGel XV RAM resin was obtained from Rapp Polymer (Tuebingen, Germany). Fmoc-protected amino acids, 5(6)-carboxyfluorescein (5,6-FAM) and coupling reagents, 1-hydroxybenzotriazole (HOBt), 2-(6-chloro-1H-benzotriazol-1-yl)-1,1,3,3-tetramethylaminium hexafluorophosphate (HCTU), and benzotriazole-1-yloxytripyrrolidinophosphonium hexafluorophosphate (PYBOP), for peptide synthesis were purchased from Chem-Impex (Wood Dale, IL). N, N’-Diisopropylethylamine (DIPEA) and N, N’-Diisopropylcarbodiimide (DIC) were purchased from Acros Organics (Thermo Fisher Scientific, Waltham, MA). Fmoc-NH-(PEG)₂-COOH (20 atoms) was purchased from EMD Millipore (Billerica, MA). Trifluoroacetic acid (TFA), thioanisole, and HPLC grade solvents (DCM, DMF, acetonitrile, and water) were purchased from Fisher Scientific (Atlanta, GA) or Sigma-Aldrich (St. Louis, MO). Asialofetuin (ASF) and iodine were purchased from Sigma-Aldrich (St. Louis, MO). Fucoidan was purchased from Toronto Research Chemicals (Ontario, Canada). Fluorescein-labelled lectins (AAL and SBA), SBA-Alexa Fluor (488 conjugate), and BSA-conjugated monosaccharides (L-fucose, D-galactose, and N-acetyl-D-galactosamine) were purchased from Vector Labs (Burlingame, CA). The monosaccharides were modified by vinyl sulfone at the anomeric carbon and subsequently conjugated to BSA.^{[51 ,52 ]}

TentaGel XV RAM resin was obtained from Rapp Polymer (Tuebingen, Germany). Fmoc-protected amino acids, 5(6)-carboxyfluorescein (5,6-FAM) and coupling reagents (HOBt, HBTU, PyBOP) were purchased from Chem-Impex (Wood Dale, IL) or Novabiochem (Gibbstown, NJ, USA). DIC was purchased from Acros Organics (Thermo Fisher Scientific, Waltham, MA). Fmoc-NH-(PEG)₂-COOH (20 atoms) was purchased from EMD Millipore (Billerica, MA). Kaiser test was purchased from AnaSpec (Fremont, CA). All solvents were purchased from Fisher Scientific (Atlanta, GA) or Sigma-Aldrich (St. Louis, MO), and were high-performance liquid chromatography (HPLC) grade. Fetuin, ASF, BME and iodine were purchased from Sigma-Aldrich (St. Louis, MO). Human cell lines were purchased from American Type Culture Collection (ATCC, Manassas, VA). Control antibiotics (penicillin and streptomycin) and enzymes (neuraminidase from Clostridium perfringens) were purchased from Sigma-Aldrich (St. Louis, MO). Cell culture media and PBS buffers were purchased from Fisher Scientific (Pittsburg, PA). FITC-labeled lectins (AAL, UEA-I, and SNA) and BSA-conjugated monosaccharides (L-fucose, D-glucose, D-galactose and L-galactose) were purchased from Vector Labs (Burlingame, CA). Cell Titer-Glo Luminescent Cell Viability Assay was purchased from Promega (Madison, WI).