BXPC-3, PANC-1 and SW1990 (all from Shanghai Genechem Co., Ltd., Shanghai, China) pancreatic cancer cell lines were cultured at 37°C in an atmosphere containing 5% CO2 in RMPI-1640 (Hyclone; Thermo Fisher Scientific, Inc.) or DMEM, supplemented with 10% FBS. Subsequently, CD44+CD24+ cells were sorted from the three cell lines using flow cytometry as previously described (19 (link),20 (link)).
Sw1990
Manufactured by Genechem
Sourced in China
The SW1990 is a laboratory centrifuge designed for high-speed sample separation. It features a robust construction and can accommodate a range of rotor configurations to handle various sample volumes and types. The SW1990 is suitable for a variety of applications in research and clinical laboratories.
Automatically generated - may contain errors
Lab products found in correlation
Panc 1, by Genechem (5 mentions)
Cf pac1, by Genechem (3 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (3 mentions)
Bxpc 3, by Genechem (2 mentions)
Trypsin, by Thermo Fisher Scientific (2 mentions)
Colo357, by Genechem (2 mentions)
Penicillin and streptomycin, by Solarbio (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Panc 1, by Thermo Fisher Scientific (1 mentions)
Sw1990, by Thermo Fisher Scientific (1 mentions)
Mem medium, by Thermo Fisher Scientific (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Aspc 1, by Genechem (1 mentions)
5 protocols using sw1990
1
Isolation of Pancreatic CSCs
2
Cell Culture of Pancreatic Cell Lines
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The human pancreatic cancer cell lines (PANC-1, SW1990, COLO357 and CF-PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China). Cells were cultured in Dulbecco's modified Eagle's medium (DMEM) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) was supplemented with 10% fetal bovine serum (FBS) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) and 1% penicillin and streptomycin (Solarbio, Beijing, China), and they were maintained in a 37 incubator containing 5% CO 2 . The medium was replaced every 24-48 h according to the cell density. Cells were observed under an inverted microscope and were digested with 0.25% trypsin (Gibco, Thermo Fisher Scientific, Waltham, MA,USA) to enable passaging of the cells when they reached 80% confluence.
3
Culturing Pancreatic Cell Lines
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A normal human pancreatic ductal epithelial cell line was purchased from RiboBio Co., Ltd. (Guangzhou, China), and the PDAC cell lines SW1990 and PANC-1 were purchased from Shanghai GeneChem Co., Ltd. (Shanghai, China). SW1990 and PANC-1 cells were cultured in DMEM (Gibco Company, USA). HPDE6C7 cells were cultured in MEM medium (Gibco, USA). Both media contained 10% inactivated fetal bovine serum (Gibco, USA). All cells were incubated in an incubator at 37°C and 5% CO2.
4
Pancreatic Cancer Cell Line Cultivation
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The human pancreatic cancer cell lines (PANC‐1, SW1990, COLO357 and CF‐PAC1) and the human pancreatic ductal cell line (HPDE) were purchased from GeneChem (Shanghai, China). Cells were cultured in DMEM (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) which was supplemented with 10% foetal bovine serum (Gibco, Thermo Fisher Scientific) and 1% penicillin and streptomycin (Solarbio, Beijing, China), and they were maintained in a 37℃ incubator containing 5% CO2. The medium was replaced every 24‐48 hours according to the cell density. Cells were observed under an inverted microscope and were digested with 0.25% trypsin (Gibco, Thermo Fisher Scientific) to enable passaging of the cells when they reached 80% confluence.
5
Cultivation of Pancreatic Cell Lines
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The human normal pancreatic ductal epithelial cell line (HPDE6‐C7) was purchased from Genio Biotechnology Co., Ltd. The human PDAC cell lines AsPC‐1, BxPC‐3, CFPAC‐1, PANC‐1, and SW1990 were purchased from Genechem Co., Ltd. These cells were cultured in complete growth medium (Gibco) containing 10% fetal bovine serum (HyClone) under humidified conditions with 95% air, 5% CO2 at 37°C according to the manufacturer's instruction.
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