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Sorpty 1750

Manufactured by Carlo Erba
Sourced in Italy

The Sorpty 1750 is a laboratory instrument used for the analysis of solid samples. It is designed to determine the specific surface area and pore size distribution of materials through adsorption and desorption of gases.

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5 protocols using sorpty 1750

1

Biomimetic Hydroxyapatite Nanocrystal Synthesis

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Biomimetic HA nanocrystals were produced as described by Nocerino et al.44 (link) Briefly, HA nanocrystals were precipitated from an aqueous solution of (CH3COO)2Ca (75 mM) by slow addition (one drop per second) of an aqueous solution of H3PO4 (50 mM), keeping the pH constant at 10 (by the addition of (NH4)OH solution). The synthesis was performed at room temperature. After this last process, the suspension of HA was washed with distilled water in order to remove ammonium ions and favor the interaction between nanocrystals.
Transmission electron microscopy (TEM) investigation was carried out using a 1200 EX microscope, linked to X-ray analysis detectors and a 3010 UHR operating at 300 kV (JEOL Ltd, Tokyo, Japan). Few droplets of the samples (in ultrapure water) were deposited on perforated carbon foils supported on conventional copper microgrids. The surface area was determined using a Sorpty 1750 instrument (Carlo Erba Reagents S.r.l., Milan, Italy) using N2 absorption at 77 K.45
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2

N2 Adsorption Characterization of Materials

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Measurements were done using a Sorpty 1750 instrument (Carlo Erba) using N2 absorption at 77 K and the well known Brunauer, Emmett, and Teller procedure [18 ].
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3

Scaffold Characterization via SEM and BET

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B-HA and S-HA scaffolds were analyzed using scanning electron microscopy (SEM). Samples were washed with saline, fixed by 2.5% glutaraldehyde and with a 1% osmium solution in phosphate buffer. The specimens were coated with colloidal gold and analyzed using scanning electron microscopy (SEM, model Stereoscan S-360, Cambridge UK) [41 (link)].
The open and total porosity of the studied ceramics was measured using Archimedes’ method and geometrical weight-volume evaluation, respectively. The specific surface area (SSA) of the scaffold was measured using the nitrogen adsorption method, following the Brunauer–Emmett–Teller (BET) model (Sorpty 1750, Carlo Erba, Milan, Italy).
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4

Surface Area Analysis by BET

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Measurements were done using a Sorpty 1750 instrument (Carlo Erba) using N2 absorption at 77 K and the well known Brunauer, Emmett, and Teller procedure.31
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5

Brunauer-Emmett-Teller Surface Area

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Measurements were performed using a Sorpty 1750 instrument (Carlo Erba) using N 2 absorption at 77 K and the well-known Brunauer, Emmett, and Teller procedure 40
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