Nod cg prkdcscid il2rgtm1sug jictac
NOD.Cg-Prkdcscid Il2rgtm1Sug/JicTac is a type of immunodeficient mouse model developed by Taconic Biosciences. It is a genetically modified mouse strain that lacks functional T cells, B cells, and natural killer cells, making it a useful tool for research involving engraftment of human cells and tissues.
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18 protocols using nod cg prkdcscid il2rgtm1sug jictac
NOG Mouse Bladder Organoid Implantation
Adoptive T-cell Transfer in Melanoma Xenograft Model
NOG mice were inoculated with 2.5 × 106 cells of the human melanoma cell line, FM82 (ESTDAB-27), subcutaneous (sc.) on the left flank. Tumor growth was measured with calipers twice a week, and represented as tumor surface (mm2). For studies of tumor homing, animals were treated with tail vein administration of 15 × 106 MAGE-A3 specific Mock or CXCR2 transduced T cells when tumor size reached approx. 30 mm2. ACT was combined with intra peritoneal (ip.) injection of 6 × 105 IU rh-IL2 at the day of ACT and twice daily for the subsequent two days. For study of tumor homing, blood was collected from the mandibular vein immediately prior to culling by cervical dislocation 7 days after ACT. Tumor, spleen, lungs and bone marrow were resected and kept in RPMI 1640 + 1% penicillin/streptomycin (Gibco) on ice until processing.
Establishment of Colorectal Tumor PDX Models
Establishing Patient-Derived Xenograft Models
Xenograft Transplantation of Human HSPCs
Xenograft Tumor Growth Measurement
Xenograft Model of Leukemia
Xenograft Mouse Model Development
Tissue specimens were aseptically cut into 1–2 mm3 pieces in a laminar hood using razor blades. Tumor pieces were implanted subcutaneously into the inter-scapular fat pads of immunodeficient mice. A total of 33 mice were supplemented with 20–25 mg Te propionate pellets, which were pressed in-house from Te-propionate powder (Sigma-Aldrich). Nineteen mice were supplemented with 12.5 mg 5α-DHT pellets (Innovative Research of America, FL, USA). Thirty-two mice received no hormone pellets.
At sacrifice, gross necropsy was performed, and the findings were documented. Tumors were removed and collected for histology or transplanted further to second or third generation of mice using the same protocol as described above.
Xenograft Model of B-ALL in NOG Mice
Murine Models for Xenograft Studies
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