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Anti arg1 fitc

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Anti-Arg1-FITC is a fluorescently labeled antibody that specifically binds to the arginase-1 (Arg1) protein. Arg1 is an enzyme involved in the urea cycle and is often used as a marker for certain cell types. The FITC label allows for detection of Arg1-positive cells or tissues using fluorescence-based techniques.

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Lab products found in correlation

Lsrfortessa flow cytometer, by BD (1 mentions) Anti cd45 pe cy7, by Thermo Fisher Scientific (1 mentions) Anti inos pe, by Thermo Fisher Scientific (1 mentions) Cytofix cytoperm kit, by Thermo Fisher Scientific (1 mentions) Collagenase, by Merck Group (1 mentions) Anti il 10 apc, by Thermo Fisher Scientific (1 mentions) M1 70, by Thermo Fisher Scientific (1 mentions) Mp5 20f3, by Thermo Fisher Scientific (1 mentions) Anti il1β apc, by Thermo Fisher Scientific (1 mentions) Gentlemacs tissue dissociator, by Miltenyi Biotec (1 mentions)

3 protocols using anti arg1 fitc

1

Intracellular Staining of iNOS and Arg1

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Cells were collected by gentle trypsinization, washed with FACS buffer, fixed with the eBioscience Fixation/Permeabilization buffer for 15 min at RT and washed once in 1× Permeabilization buffer. For intracellular staining, cells were stained with anti-iNOS-APC/Cy7 antibody (eBioScience) and anti-Arg1-FITC (R&D) for 30 min at RT. After washing once in 1× Permeabilization buffer, cells were resuspended in FACS buffer and run on a BD LSRFortessa Flow Cytometer (BD, NJ). Data were analyzed with FlowJo (Treestar, OR).
Herr D.R., Yam T.Y., Tan W.S., Koh S.S., Wong W.S., Ong W.Y, & Chayaburakul K. (2020). Ultrastructural Characteristics of DHA-Induced Pyroptosis. Neuromolecular Medicine, 22(2), 293-303.
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2

Microglia iNOS and Arg-1 Expression

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To determine the expression of inducible nitric oxide synthase (iNOS) and arginase-1 (Arg-1) by microglia, brain mononuclear cells were harvested as described in previous section. Cells were surface stained using anti-CD45-PE-Cy7 and anti-CD11b-BV421 prior to fixation/permeabilization using cytofix/cytoperm kit (eBioscience). Cells were then stained with anti-iNOS-PE (eBioscience) and anti-Arg-1-FITC (R&D Systems), as recommended by manufacturer’s protocol.
Chauhan P., Hu S., Sheng W.S., Prasad S, & Lokensgard J.R. (2017). Modulation of Microglial Cell Fcγ Receptor Expression Following Viral Brain Infection. Scientific Reports, 7, 41889.
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3

Flow Cytometric Analysis of Colon Macrophages

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Mouse colon tissues were treated with collagenase (I, II, and IV, Sigma Aldrich) and dispersed using the gentleMACs tissue dissociator (Miltenyi Biotech, Cologne, Germany). Cell suspensions were incubated overnight in media before staining for flow cytometry according to standard Biolegend protocols (Biolegend, San Diego, CA). Macrophages were stained with anti-F4/80-PE or FITC (Biolegend, BM8), anti-CD11b-APC or PE (eBioscience M1/70), anti-IL-1α-PE (eBioscience, ALF-161), anti-IL-1β-APC (eBioscience NJTEN3), anti-IL6 (eBioscience MP5-20F3), anti-TNF-α-PEcy5 (MP6-XT22 eBioscience), anti-IL-10-APC (eBioscience JES5-16E3), anti-Arg1-FITC (R&D Systems IC5868F) or isotype controls. All samples were run on a Guava easyCyte 8HT flow cytometer. Cells were gated on the forward and side scatter plot to remove debris, next on the F4/80+CD11b+ population and examined for cytokines. Macrophage numbers per colon were calculated by the percent of gated cells in relation to the overall number of cells per mouse colon.
Ray A.L., Castillo E.F., Morris K.T., Nofchissey R.A., Weston L.L., Samedi V.G., Hanson J.A., Gaestel M., Pinchuk I.V, & Beswick E.J. (2015). Blockade of MK2 is protective in inflammation-associated colorectal cancer development. International journal of cancer. Journal international du cancer, 138(3), 770-775.
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