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Vic fluorescent dye

Manufactured by Thermo Fisher Scientific
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VIC fluorescent dye is a nucleic acid stain used in molecular biology applications. It is a commonly used reporter dye in TaqMan assays and other real-time PCR techniques.

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Lab products found in correlation

Rneasy mini kit, by Qiagen (2 mentions) Abi prism 7700 user bulletin, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

Fluorescent dyes (25 citations) VIC™ dye (3 citations)

2 protocols using vic fluorescent dye

1

Quantitative Gene Expression Analysis

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Cellular RNA was extracted with the RNeasy mini kit (Qiagen) and was reverse-transcribed into cDNA by the ABI PRISM TaqMan reverse transcription method. Expression of genes of interest and of Gapdh was assessed by Simplex RT–PCR with probes labelled with 5-carboxyfluorescein and VIC fluorescent dye (Applied BioSystems). All the probe sets were from Applied BioSystems (Supplementary Table 1). Transcription of target genes was calculated according to the 2−ΔΔCT method as described by the manufacturer (ABI PRISM 7700 user bulletin; Applied BioSystems) and was presented in arbitrary units as previously reported49 (link).
Xiao X., Shi X., Fan Y., Zhang X., Wu M., Lan P., Minze L., Fu Y.X., Ghobrial R.M., Liu W, & Li X.C. (2015). GITR subverts Foxp3+ Tregs to boost Th9 immunity through regulation of histone acetylation. Nature Communications, 6, 8266.
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2

Gene Expression Analysis by RT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular RNA was extracted with the RNeasy mini kit (Qiagen) and was reverse-transcribed into cDNA by the ABI PRISM TaqMan reverse transcription method. Expression of genes of interest and of Gapdh was assessed by Simplex RT-PCR with probes labeled with 5-carboxyfluorescein and VIC fluorescent dye (Applied BioSystems). All the probe sets were from Applied BioSystems (Supplementary Table 1). Transcription of target genes was calculated according to the 2−ΔΔCT method as described by the manufacturer (ABI PRISM 7700 user bulletin; Applied BioSystems) and was presented in arbitrary units as previously reported49 (link).
Xiao X., Shi X., Fan Y., Zhang X., Wu M., Lan P., Minze L., Fu Y.X., Ghobrial R.M., Liu W, & Li X.C. (2015). GITR subverts Foxp3+ Tregs to boost Th9 immunity through regulation of histone acetylation. Nature Communications, 6, 8266.
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