Anti vglut1

For immunohistochemical analysis, cells were fixed with 4% paraformaldehyde (PFA) in 120mM phosphate buffer (PBS), pH 7.4, permeabilized with 0.05% Triton X-100 in PBS, blocked with 10% goat serum in PBS and subjected to immunohistochemistry staining with primary and secondary antibodies diluted in the blocking solution.
For immunolabelling the following antibodies at indicated dilutions were used: anti-Nestin (1:400; BD Bioscience), anti-Sox1 (1:100; R&D Systems), anti-Sox2 (1:200; Abcam), anti-Pax6 (1:200; DSHB), anti-Ki67 (1:200; Vector Labs), anti-TuJ1 (1:400; Covance), anti-Map2 (1:200; Millipore), anti-DCX (1:200; Millipore), anti-GFAP (1:200; Millipore), anti-O4 (1:50, Sigma-Aldrich), anti-GABA (1:200, Abcam), anti-vGlut1 (1:200; Millipore), anti-TH (1:100, Millipore), anti-Synaptophysin (1:100; Millipore), anti-PSD95 (1:200; Invitrogen), anti-vimentin (1:5000; Abcam), anti-S100β (1:1000; Sigma-Aldrich), anti-aquaporin 4 (AQP4, 1:100; Santa Cruz Biotechnology) and anti-excitatory amino acid transporter 2 (EAAT2, 1:100; Santa Cruz Biotechnology), secondary Alexafluorophore-conjugated antibodies (1:1000, Invitrogen). DNA was stained using Hoechst 33258 (1:10000, Invitrogen). All cells expressing a particular marker were counted manually and normalized to the total number of cells.

Immunostaining of brain sections was performed as described previously^{61 (link),62 (link)}. The following primary antibodies were used: anti-Parvalbumin (Millipore, Rabbit, AB1572; 1:200), anti-Arid1b (Abcam, Mouse, ab57461; 1:200), anti-VIAAT (PhosphoSolutions, Rabbit, 2100-VGAT, 1:500), anti-VGLUT1 (Millipore, Guinea pig, AB5905, 1:1000), GAD2 (Developmental Studies Hybridoma Bank, Rat, GAD6, 1:200), and anti-Somatostatin (MilliporeSigma, Rat, MAB354; 1:200). 4′,6-diamidino-2-phenylindole (DAPI; Sigma; 1μg/ml) was used for counterstaining. Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies. All slides were visualized and imaged under FV3000 (Olympus) fluorescent confocal microscope system. To quantify ARID1B expression levels, we measured the intensity of ARID1B fluorescence in PV or SST positive cells using NIH ImageJ. The expression intensities were averaged and presented after background subtraction and normalization to those of PV or SST negative cells.

To characterize iPSC-derived neuronal cultures, neurons were fixed with 3.7% FA in 4% sucrose 14 days after the start of differentiation (d14). After blocking and permeabilization, neurons were stained with primary and secondary antibodies as described before [41 ]. The following antibodies were used: anti-TAU (rabbit, K9JA, Dako (Jena, Germany), A0024), anti-MAP2 (chicken, Abcam (Camebridge,. UK), ab5392), anti-vGlut1 (mouse, Merck Millipore (Burlington, MA, USA), MAB5502), anti-ChAT (rabbit, Thermofisher Scientific, PA5-26597), anti-SYP (mouse, Santa Cruz Biotechnology (Dallas, TX, USA), sc-17750), anti-vGlut1 (rabbit, Synaptic Systems (Göttingen, Germany), 135303), anti-PSD95 (mouse, Biolegend (San Diego, CA, USA), MMS-5182), anti-rabbit IgG Alexa-Fluor-488 (donkey, Thermofisher Scientific, A21206), anti-mouse IgG Alexa-Fluor-488 (donkey, Thermofisher Scientific, A21202), anti-chicken IgG AlexaFluor-647 (goat, Thermofisher Scientific, A21449), anti-rabbit IgG AlexaFluor-568 (goat, Thermofisher Scientific, A11011), anti-mouse IgG AlexaFluor-568 (donkey, Thermofisher Scientific, A10037).

For immunohistochemistry, guinea pig polyclonal anti-VGLUT1 and anti-VGLUT2 were purchased from Merck Millipore (#AB5905 and #AB2251-I, respectively, Bilerica, MA, USA). anti-VGLUT1 and anti-VGLUT2 were produced against the peptides corresponding to the C-terminus of rat VGLUT1 and VGLUT2. Mouse monoclonal antibody anti-microtubule-associated protein 2 (MAP2) was obtained from Sigma (#M 4403, clone HM-2, St. Louis, MO, USA) and raised against rat brain MAP2.
For the Western blot, mouse monoclonal anti-PSD-95 was obtained from Merck Millipore (#MAB1596, Bilerica, MA, USA). The antibody was raised against a recombinant rat PSD-95 protein. A mouse monoclonal antibody to Glyceraldehyde-3-phosphate dehydrogenase (anti-GAPDH) from Thermo Fisher Scientific (#AM4300, clone 6C5, Madrid, Spain) was used as a load control.