CPT was purchased from Sigma-Aldrich (≥98% purity; St. Louis, MO, USA) and dissolved in dimethyl sulfoxide (DMSO) as a stock solution at 0.1 M. Chloroquine (CQ) and 3-Methyladenine (3-MA) were purchased from Sigma-Aldrich. Insulin-like growth factor-I (IGF-I) was obtained from R&D Systems, Inc. (Minneapolis, MN, USA). Annexin V-FITC/PI Apoptosis Detection Kit was purchased from Vazyme (Nanjing, China). BeyoClick™ EdU-555 Cell Proliferation Assay Kit was purchased from Beyotime (Shanghai, China). Antibodies, including PI3-kinase p-p85-α (ab182651), Bcl-2 (ab59348), HRP-conjugated goat anti-rabbit (ab6721), and anti-mouse IgG (ab6789) were purchased from Abcam Co. (Cambridge, MA, USA). Antibodies, including AKT (#9272), p-AKT (#4058), Bax (#2772), PARP (#9532), Caspase-3 (#9662), Cleaved Caspase-3(#9664), LC3B (#3868), Beclin-1 (#3495), SQSTM1/p62 (#8025), and β-actin (ab8227) were obtained from Cell Signaling Technology Inc. Antibodies, including anti-PI3-kinase p85-α (SAB4502195), were purchased from Sigma-Aldrich.
Anti mouse igg ab6789
Manufactured by Abcam
Sourced in United Kingdom
Anti-mouse IgG (ab6789) is a secondary antibody that binds to mouse immunoglobulin G (IgG) antibodies. It is designed for use in various immunoassays and research applications.
Automatically generated - may contain errors
Lab products found in correlation
β actin, by Cell Signaling Technology (2 mentions)
Insulin like growth factor 1 igf 1, by R&D Systems (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
Chloroquine cq, by Merck Group (1 mentions)
P akt, by Cell Signaling Technology (1 mentions)
Sqstm1 p62, by Cell Signaling Technology (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Vazyme (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Beyoclick edu 555 cell proliferation assay kit, by Beyotime (1 mentions)
Caspase 3, by Cell Signaling Technology (1 mentions)
Gapdh, by Cell Signaling Technology (1 mentions)
Bicinchoninic acid protein assay kit, by BestBio (1 mentions)
Sc 57315, by Santa Cruz Biotechnology (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
3 protocols using anti mouse igg ab6789
1
Exploring Autophagy and Apoptosis Mechanisms
2
Western Blot Analysis of IL-6 Protein
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Protein was extracted using radioimmunoprecipitation assay buffer (50 mM Tris HCl, pH 7.4, 150 mM NaCl, 1% Nonidet P40 and 0.1% sodium dodecyl sulfate) and phenylmethanesulfonyl fluoride at 4°C. Bicinchoninic acid protein assay kit (BestBio, Shanghai, China) was used to determine the concentration. The concentration of each sample was 500 ng/µl and all the samples were run on 10% sodium dodecyl sulfate-polyacrylamide gel and electro-transferred onto polyvinylidene fluoride membranes for 1 h at 4°C. After being electro-transferred, the polyvinylidene fluoride membranes were blocked by skimmed milk for 1 h at room temperature. Antibodies against GAPDH (97166; 1:4,000; Cell Signaling Technology, Inc., Danvers, MA, USA) and IL-6 (sc-57315; 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA) were incubated with blots overnight at 4°C. Then the blots were incubated with secondary antibody (anti-mouse IgG; ab6789; 1:5,000; Abcam, Cambridge, UK) at room temperature for 1 h. Proteins were detected by electrochemiluminescence (Beijing Dingguo Changsheng Biotechnology Co., Ltd., Beijing, China).
3
Molecular Signaling Pathway Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Fetal bovine serum (FBS), Dulbecco’s modified Eagle’s medium (DMEM), and penicillin–streptomycin were provided from Gibco. Cell culture supplies were obtained from Costar (Corning, USA). Antibodies, including anti-PI3-kinase p85-α (ab182651), anti-Bcl-2 (ab59348), HRP-conjugated goat anti-rabbit (ab6721), and anti-mouse IgG (ab6789) were from Abcam (Cambridge, UK). Antibodies, including anti-EGFR (#4267), anti-Bax (#2772), anti-AKT (#9272), anti-p-AKT (#4058), and β-actin (ab8227) were obtained from Cell Signaling Technology (Boston, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!