Alveole primo

Manufactured by Nikon

Sourced in Japan

The Alveole Primo is a lab equipment product designed for controlled sample environment. It provides precise temperature, humidity, and gas flow regulation to facilitate reliable scientific experimentation. The device's core function is to create and maintain a stable environmental setting for various experimental applications.

Automatically generated - may contain errors

Lab products found in correlation

Sola se 2 365, by Lumencor (2 mentions) Orca flash 4.0 lt b w camera, by Hamamatsu Photonics (2 mentions)

2 protocols using alveole primo

Imaging Microfluidic Chip Fluorescence

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Chips were imaged under a fluorescence microscope Nikon Ti-E with Alveole Primo (Nikon, Tokyo, Japan) connected to Hamamatsu Orca Flash 4.0 LT B&W camera (Hamamatsu Photonics, Hamamatsu, Japan) and Lumencor Sola SE II 365 (Lumencor, Beaverton, Oregon, USA). Images for the following transmitted light and fluorescent filters were acquired: DAPI (Semrock 5060C, excitation 377/50, emission 447/60), GFP (Semrock 3035D-NTE, excitation 472/30, emission 520/35), TxRed (Semrock 4040C, excitation 531/40, emission 593/40), and Cy5 (excitation 640/20, emission 700/75). Multiple chips were placed on a microscope slide adapter. The NIS-Elements Advanced Research program was automated to image at 20x magnification and to scan 10 images horizontally to form a complete representation of the channel producing a multichannel composite image. Images of the chips were acquired daily to confirm opening of the microchannels after 24 hours of incubation (Figure 1D).

Sieviläinen M., Saavalainen J., Adnan-Awad S., Salo T, & Al-Samadi A. (2022). IDO1 Inhibition Reduces Immune Cell Exclusion Through Inducing Cell Migration While PD-1 Blockage Increases IL-6 and -8 Secretion From T Cells in Head and Neck Cancer. Frontiers in Immunology, 13, 812822.

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Automated Imaging of Microfluidic Chips

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chips were imaged daily under a fluorescent microscope using Nikon Ti-E with Alveole Primo (Nikon, Tokyo, Japan) connected to a Hamamatsu Orca Flash 4.0 LT B&W camera (Hamamatsu Photonics, Hamamatsu, Japan) and Lumencor Sola SE II 365 (Lumencor, Beaverton, USA). Images for the following transmitted light and fluorescent filters were acquired: DAPI (Semrock 5060C, excitation 377/50, emission 447/60), GFP (Semrock 3035D-NTE, excitation 472/30, emission 520/35), and Cy5 (excitation 640/20, emission 700/75). Chips were placed on a microscope slide adaptor. The NIS-Elements Advanced Research program was automated to image at 20x magnification and to scan 10 images horizontally to form a complete representation of the channel producing a multichannel composite image.

Wahbi W., Korelin K., Sieviläinen M., Karihtala P., Wilkman T., Tarkkanen J., Salo T, & Al-Samadi A. (2023). Evaluation of in vitro and in vivo personalized cancer treatment assays for oral squamous cell carcinoma. Translational Oncology, 33, 101677.

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