The following primary antibodies were used in this study: anti-LRRK2 (MJFF2 [c41-2]; Abcam), anti-human LAMP1 (D2D11; Cell Signaling Technology), anti-mouse LAMP1 (1D4B; Bio-Rad), anti-LC3 (PM036; MBL), anti-LC3 (L7543; Sigma-Aldrich), anti-GFP (A-11122; Invitrogen), anti-p62 (61832; BD Pharmingen), anti-galectin-3 (M3/38; BioLegend), anti-α-tubulin (DM1A; Sigma-Aldrich), anti-CatD (ERP3057Y; ab75852), anti-CatB (D1C7Y; Cell Signaling Technology), anti-Rab10 (D36C4; Cell Signaling Technology), anti-phospho-Thr73 Rab10 (MJF-R21 [ab230261]; Abcam), anti-ATG16L1 (D6D5; CST), anti-ATG5 (A0731; Sigma-Aldrich), anti-FIP200 (17250-1-AP; Proteintech), anti-ATG13 (SAB4200100; Sigma-Aldrich), and anti-Rab29 (MJF-R30-124; Abcam). The following reagents were used at final concentrations as indicated: CQ (50–100 μM; Sigma-Aldrich), LLOMe (1 mM; Sigma-Aldrich), and zymosan (Sigma-Aldrich).
Sab4200100
Manufactured by Merck Group
The SAB4200100 is a laboratory equipment product manufactured by Merck Group. It is designed for use in scientific research and testing applications. The core function of this product is to provide a controlled and stable environment for various laboratory procedures.
Automatically generated - may contain errors
Lab products found in correlation
Anti gfp a 11122, by Thermo Fisher Scientific (1 mentions)
Zymosan, by Merck Group (1 mentions)
Anti α tubulin dm1a, by Merck Group (1 mentions)
Anti fip200 17250 1 ap, by Proteintech (1 mentions)
Llome, by Merck Group (1 mentions)
Anti atg5 a0731, by Merck Group (1 mentions)
Anti lc3 l7543, by Merck Group (1 mentions)
α tubulin, by Merck Group (1 mentions)
Alexa fluor 568 donkey anti mouse, by Thermo Fisher Scientific (1 mentions)
α gfp 3h9, by Proteintech (1 mentions)
Nbp243792, by Novus Biologicals (1 mentions)
Sab2102258, by Merck Group (1 mentions)
Sab2102257, by Merck Group (1 mentions)
Ab8245, by Abcam (1 mentions)
2 protocols using sab4200100
1
Antibody Validation for LRRK2 and Lysosomal Proteins
2
Immunoblotting and Immunofluorescence Antibody Panel
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The following primary antibodies were used for immunoblotting and immunofluorescence: α-Actin (A5316, Sigma Aldrich), α-ATG3 (3415, CST), α-ATG101 (SAB4200175, Sigma-Aldrich), α-ATG13 (M183-3, MBL; SAB4200100, Sigma-Aldrich), α-ATG14 (PD026, MBL), α-ATG14 pS29 (92340, CST), α-Coilin (PA5-29531, Invitrogen), α-FIP200 (A301-574A, Bethyl), α-GAPDH (ab8245, Abcam), α-GFP (3H9, Chromotek), α-LC3B (2775, CST), α-p62 (GP62-C, PROGEN), α-pICln (sc-393525, Santa Cruz), α-PRMT5 (2252, CST), α-SmB (S0698, Sigma-Aldrich), α-SmD1 (ab79977, Abcam), α-SmD2 (SAB2102257, Sigma-Aldrich), α-SmE (NBP2-43792, Novus), α-SmF (SAB2102258, Sigma-Aldrich), α-SmG (HPA064152, Sigma-Aldrich), α-SMN (clone 2B1, 05-1532, Merck Millipore), α-SNRPB (Y12, MA5-13449, Invitrogen), α-Tubulin (clone B512, T5168, Sigma Aldrich), α-ULK1 (8054; CST), α-ULK2 (ab97695, Abcam), α-WD45 (2823, CST). The detection of proteins was carried out with the following fluorescent secondary antibodies: IRDye 680LT goat α-rabbit, IRDye 680LT goat α-mouse, IRDye 800CW donkey α-rabbit, IRDye 800CW donkey α-mouse, IRDye 800CW goat α-rat. For the detection of proteins in vivo via IF the following secondary antibodies were used: Alexa Fluor 568 donkey anti-mouse (A10037, Invitrogen) and Alexa Fluor 647 donkey anti-rabbit (A31573, Invitrogen).
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