Stemdiff intestinal organoid growth medium

The differentiation from iPSCs into intestinal organoids were performed with STEMdiff Intestinal Organoid Kit (STEMCELL) according to the manufacturer’s instructions. The human intestinal organoids were cultured in STEMdiff Intestinal Organoid Growth Medium (STEMCELL) in Matrigel. The organoids were differentiated into GCs with the addition of IWP2 (1.5 mM; Sigma Aldrich, cat: I0536) and DAPT (10 mM, Sigma Aldrich, cat: D5942) or A83–01 (1 mM; Cayman, cat: 9001799) and DBZ (10 mM; Cayman, cat: 14627) in Organoid Growth Medium.

The human iPSC line ND1.4 derived from human fibroblast was got from iPSC core in National Heart, Lung, and Blood Institute (NHLBI). The iPSCs were maintained in the feeder free Essential 8™ Medium (Thermo Fisher) on Matrigel. The differentiation from iPSCs into intestinal organoids were performed with STEMdiff™ Intestinal Organoid Kit (Stemcell) according to the manufacturer’s instructions. The human intestinal organoids were cultured in STEMdiff™ Intestinal Organoid Growth Medium (Stemcell) in Matrigel. The organoids were differentiated into EEC with the addition of IWP2 (1.5 mM; Sigma Aldrich), DAPT (10 mM, Sigma Aldrich) and MEK inhibitor PD0325901 (1 mM; Sigma Aldrich) in media. All human subjects studies were performed under signed consent and approved by the NIH Review Board (IRB), PRD #7169.