Lipofectatimine 2000

Manufactured by Thermo Fisher Scientific

Lipofectamine 2000 is a transfection reagent used for the delivery of nucleic acids, such as plasmid DNA, mRNA, or siRNA, into mammalian cells. It is a cationic lipid-based formulation that facilitates the uptake of these molecules into the cells.

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Lab products found in correlation

Polybrene, by Merck Group (2 mentions) 8 br cgmp, by Merck Group (2 mentions) Kt5823, by Cayman Chemical (2 mentions) Riociguat, by Selleck Chemicals (2 mentions) Ns2028, by Cayman Chemical (2 mentions)

2 protocols using lipofectatimine 2000

Modulating cGMP Signaling Pathways

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siRNA against ERG, GUCY1A1, GUCY1B1, non-target control (NTC) were purchased from Dharmacon and transfected into cells using lipofectatimine 2000 (Thermo Fisher). VCaP cells were infected with lenti-virus containing shRNAs against ERG or NTC (Dharmacon) and mixed with polybrene (Millipore) following manufacture’s protocol, and further selected in medium containing puromycin. The small molecule treatments were listed as following: ODQ (Sigma), riociguat (Selleck), NS2028 (Cayman), KT5823 (Cayman) and 8-Br-cGMP (Sigma).

Zhou F., Gao S., Han D., Han W., Chen S., Patalano S., Macoska J.A., He H.H, & Cai C. (2019). TMPRSS2-ERG activates NO-cGMP signaling in prostate cancer cells. Oncogene, 38(22), 4397-4411.

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Targeting cGMP Signaling in Cancer

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siRNA against ERG, GUCY1A1, GUCY1B1, NTC were purchased from Dharmacon and transfected into cells using lipofectatimine 2000 (Thermo Fisher). VCaP cells were infected with lenti-virus containing shRNAs against ERG or NTC (Dharmacon) and mixed with polybrene (Millipore) following manufacture’s protocol, and further selected in medium containing puromycin. The small molecule treatments were listed as following: ODQ (Sigma), riociguat (Selleck), NS2028 (Cayman), KT5823 (Cayman) and 8-Br-cGMP (Sigma).

Zhou F., Gao S., Han D., Han W., Chen S., Patalano S., Macoska J.A., He H.H, & Cai C. (2019). TMPRSS2-ERG activates NO-cGMP signaling in prostate cancer cells. Oncogene, 38(22), 4397-4411.

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