Nuciferine

Manufactured by Yuanye Bio-Technology

Sourced in China

Nuciferine is a laboratory equipment product offered by Yuanye Bio-Technology. It is a chemical compound used for analytical and research purposes. The core function of Nuciferine is to serve as a reference standard or analytical tool in various scientific applications.

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Lab products found in correlation

Milli q water purification system, by Merck Group (1 mentions) Methanol, by Sinopharm (1 mentions) Ethyl acetate, by Sinopharm (1 mentions) Petroleum ether, by Sinopharm (1 mentions) Hplc grade acetonitrile, by Tedia (1 mentions) N nornuciferine, by Yuanye Bio-Technology (1 mentions) Hplc dad, by Shimadzu (1 mentions) Milli q system, by Merck Group (1 mentions)

3 protocols using nuciferine

Alkaloid Extraction and Quantification

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Alkaloid extraction and separation methods used
herein were the same as those described in a previous study.^{5 (link)} Separation and detection were performed by HPLC-DAD
(SHIMADZU) using a TOSOH C18 column (4.6 mm × 150 mm, 3.5 μm
diameter). Nuciferine and N-norNuciferine alkaloid
contents were determined against the linear standard curves of Nuciferine
(Yuanye Biotechnology Shanghai, China) as a representative aporphine-type
alkaloid (Figure S2). The peak areas of
Nuciferine and N-norNuciferine were pooled to calculate
the total content of major BIAs.

Meelaph T., Kobtrakul K., Chansilpa N.N., Han Y., Rani D., De-Eknamkul W, & Vimolmangkang S. (2018). Coregulation of Biosynthetic Genes and Transcription Factors for Aporphine-Type Alkaloid Production in Wounded Lotus Provides Insight into the Biosynthetic Pathway of Nuciferine. ACS Omega, 3(8), 8794-8802.

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Lotus Leaf Powder Extraction and Nuciferine Quantification

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Lotus leaf was provided by Hangzhou Tongjuntang Co., Ltd. (Hangzhou, Zhejiang, China). It was ground and sieved. The powder with a particle size of 0.150 and 0.420 mm was used for experiments. Silica gel with a particle size of 0.075–0.150 mm was purchased from Qingdao Haiyang Chemical Co., Ltd. (Qingdao, Shandong, China). Nuciferine was purchased from Shanghai Yuanye Biotechnology Co., Ltd. (Shanghai, China). HPLC-grade acetonitrile was purchased from Tedia Company Inc (Fairfield, OH, USA). Acetic acid and triethylamine were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Analytical ammonia solution, petroleum ether, ethyl acetate, and methanol were purchased from Sinopharm chemical reagent Co., Ltd. (Shanghai, China). Deionized water was prepared by a Milli-Q water-purification system from Milipore (Bedford, MA, USA).

Ruan Y., Xu J., Chu J., Shi J, & Shi Q. (2022). Processing tactics for low-cost production of pure nuciferine from lotus leaf. Ultrasonics Sonochemistry, 86, 106026.

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Nuciferine Neuroprotection Evaluation

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Nuciferine was purchased from Shanghai yuanye Bio-Technology Company, Ltd. (purity ≥ 98%; Shanghai, China). Carboxymethylcellulose sodium (CMC-Na) was purchased from Beijing BioDee Biotechnology Company, Ltd. (Beijing, China). 2,3,5-Triphenyl-2H-Tetrazolium Chloride (TTC solution, 2%) was obtained from Beijing Solarbio Science and Technology Company, Ltd. (Beijing, China). Nissl staining kit was purchased from Beijing Beyotime Biotechnology Company, Ltd. (Beijing, China). Double distilled water was provided by a Milli-Q system (Millipore, MA, USA).

Chen C., Ma Q., Jiang J., Wang T., Qiu L, & Liu A. (2022). Protective Effects of Nuciferine in Middle Cerebral Artery Occlusion Rats Based on Transcriptomics. Brain Sciences, 12(5), 572.

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