Whole ear pinnae were homogenised in 1.5 ml flat-bottom tubes containing 400 μl of 0.01% BSA/PBS with an OMNI Tissue Homogeniser with plastic hard-tissue probes (OMNI International, Kennesaw, GA, Georgia). The homogenate was then centrifuged at 10,000 rcf for 20 min at 4 °C and the supernatant was collected and stored at -80 °C. The levels of IFNγ, TNFα, IL-1β, IL-4, IL-6, IL-10, IL-33, CCL2, CCL3, CCL4, CCL5, CCL7, CCL20, CXCL1, CXCL2, CXCL5 and CXCL10 were measured using Magnetic Luminex Mouse Premixed Multi-Analyte kits (R&D Systems, Minneapolis, Minnesota) and a Luminex 200 analyser (Luminex Corporation, Austin, Texas).
Plastic hard tissue probes
Manufactured by Omni International
Plastic hard-tissue probes are used for the examination and manipulation of hard tissue samples in a laboratory setting. They are designed to be durable and provide precise control for delicate procedures.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using plastic hard tissue probes
1
Cytokine Profiling of Murine Ear Tissue
2
Comprehensive Cytokine Profiling in Tissues
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Whole ears, dLN or lungs were homogenized in 1.5 ml flat-bottom tubes containing 400 μl of 0.5% BSA/PBS using an OMNI Tissue Homogenizer with plastic hard tissue probes (OMNI International). The tissue homogenates were centrifugated at 10,000 rcf for 20 min, at 4°C and supernatants were obtained and stored at −70°C. Magnetic Luminex Mouse Premixed Multi-Analyte kits were purchased from R&D Systems, to assess levels of IFNγ, TNFα, IL-1β, IL-4, IL-6, IL-10, IL-33, CCL2, CCL3, CCL4, CCL5, CCL7, CCL20, CXCL1, CXCL2, CXCL5 (LIX), and CXCL10 using a Luminex 200 analyzer (Luminex Corporation).
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