Log In Sign up
The largest database of trusted experimental protocols

Halo 2.3 platform

Manufactured by Indica Labs
Visit Supplier

The HALO 2.3 platform is a high-performance digital pathology solution developed by Indica Labs. It is designed to enable efficient analysis and management of whole slide images. The HALO 2.3 platform provides advanced image processing and analysis capabilities, facilitating the comprehensive evaluation of tissue samples.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using halo 2.3 platform

1

Quantifying Mucosal Immune Cells in Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were analyzed using the HALO 2.3 platform (Indica Labs) in a blinded fashion. All tissues were annotated to limit analysis to the mucosa (i.e. epithelium, lamina propria), including lymphoid aggregates and putative colonic patches originating within the submucosa that breached the muscularis mucosa and penetrated into the lamina propria. The submucosa and tunica muscularis were excluded from analysis. For neutrophil quantification, the percentage of MPO positive cells was determined relative to the total cell population using the Cytonuclear v1.6 module. MX1 was quantified as the percent positive staining relative to the total tissue area using the Area Quantification v2.0.0 analysis module. For ILC3 quantification, the number of CD117+CD3−, CD117−CD3+, and total cells was determined using the FISH v1.1 analysis module. All analyses were reviewed manually to ensure accuracy.
Wang Y., Lifshitz L., Gellatly K., Vinton C.L., Busman-Sahay K., McCauley S., Vangala P., Kim K., Derr A., Jaiswal S., Kucukural A., McDonel P., Hunt P.W., Greenough T., Houghton J., Somsouk M., Estes J.D., Brenchley J.M., Garber M., Deeks S.G, & Luban J. (2020). HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells. Nature immunology, 21(3), 274-286.
+ Open protocol
+ Expand
2

Quantifying Mucosal Immune Cells in Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols
Images were analyzed using the HALO 2.3 platform (Indica Labs) in a blinded fashion. All tissues were annotated to limit analysis to the mucosa (i.e. epithelium, lamina propria), including lymphoid aggregates and putative colonic patches originating within the submucosa that breached the muscularis mucosa and penetrated into the lamina propria. The submucosa and tunica muscularis were excluded from analysis. For neutrophil quantification, the percentage of MPO positive cells was determined relative to the total cell population using the Cytonuclear v1.6 module. MX1 was quantified as the percent positive staining relative to the total tissue area using the Area Quantification v2.0.0 analysis module. For ILC3 quantification, the number of CD117+CD3−, CD117−CD3+, and total cells was determined using the FISH v1.1 analysis module. All analyses were reviewed manually to ensure accuracy.
Wang Y., Lifshitz L., Gellatly K., Vinton C.L., Busman-Sahay K., McCauley S., Vangala P., Kim K., Derr A., Jaiswal S., Kucukural A., McDonel P., Hunt P.W., Greenough T., Houghton J., Somsouk M., Estes J.D., Brenchley J.M., Garber M., Deeks S.G, & Luban J. (2020). HIV-1-induced cytokines deplete homeostatic innate lymphoid cells and expand TCF7-dependent memory NK cells. Nature immunology, 21(3), 274-286.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!