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Cd40 sirna

Manufactured by GenePharma
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Sourced in China

CD40-siRNA is a laboratory product that functions as a small interfering RNA (siRNA) targeting the CD40 gene. It is designed to facilitate gene silencing experiments in biological research.

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Lab products found in correlation

Sirna mate transfection reagent, by GenePharma (2 mentions) Cd40l, by Thermo Fisher Scientific (2 mentions) E coli o111 b4, by Merck Group (2 mentions) Traf6 sirna, by GenePharma (2 mentions)

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SiRNAs (565 citations)

2 protocols using cd40 sirna

1

Induction of Enteric Glial Cell Sepsis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce the cell sepsis model, EGCs were stimulated by single lipopolysaccharide (LPS) (10 μg/mL, E. coli O111:B4, Sigma-Aldrich, St. Louis, MO, USA), CD40L (1 μg/mL, PeproTech, Rocky Hill, NJ, USA) or LPS + CD40L. After 24 h, the cell culture supernatants were collected as EGCs-conditioned medium for cytokine measurement and Caco-2 cell treatment.
For the siRNA transfection, CD40-siRNA, TRAF6-siRNA, negative control siRNA (NC-siRNA), and siRNA-Mate transfection reagent were purchased from GenePharma Co. Ltd, (Shanghai, China). EGCs were transfected with CD40-siRNA, TRAF6-siRNA or NC-siRNA using siRNA-Mate according to the manufacturer’s protocols for 72 h before LPS and CD40L stimulation.
Cheng B., Du M., He S., Yang L., Wang X., Gao H., Chang H., Gao W., Li Y., Wang Q, & Li Y. (2022). Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis. Molecular Medicine, 28, 137.
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2

Induction of Enteric Glial Cell Sepsis

Check if the same lab product or an alternative is used in the 5 most similar protocols
To induce the cell sepsis model, EGCs were stimulated by single lipopolysaccharide (LPS) (10 μg/mL, E. coli O111:B4, Sigma-Aldrich, St. Louis, MO, USA), CD40L (1 μg/mL, PeproTech, Rocky Hill, NJ, USA) or LPS + CD40L. After 24 h, the cell culture supernatants were collected as EGCs-conditioned medium for cytokine measurement and Caco-2 cell treatment.
For the siRNA transfection, CD40-siRNA, TRAF6-siRNA, negative control siRNA (NC-siRNA), and siRNA-Mate transfection reagent were purchased from GenePharma Co. Ltd, (Shanghai, China). EGCs were transfected with CD40-siRNA, TRAF6-siRNA or NC-siRNA using siRNA-Mate according to the manufacturer’s protocols for 72 h before LPS and CD40L stimulation.
Cheng B., Du M., He S., Yang L., Wang X., Gao H., Chang H., Gao W., Li Y., Wang Q, & Li Y. (2022). Inhibition of platelet activation suppresses reactive enteric glia and mitigates intestinal barrier dysfunction during sepsis. Molecular Medicine, 28, 127.
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