Moorflpi 2 blood flow imager

Renal microvascular flow was assessed using the laser speckle contrast approach. Briefly, the left kidney was exposed and scanned with a moorFLPI-2 blood flow imager and renal microcirculation was quantified using the imager's analysis software (Moor Instruments, Wilmington, DE). Throughout the measurement, mice were under anesthesia using 1.5% isoflurane. Core body temperature was monitored and maintained at 37.2 ± 0.1°C.

After 12 h of fasting, all rats were anesthetized with intraperitoneal injection of 3% pentobarbital (0.1 ml/100 g body weight). After laparotomy, we first used the moorFLPI-2 blood flow imager (Moor Instruments, UK) to scan the surface of the liver for blood flow. The distance between the scan head and the liver surface was approximately 20 cm. The image acquisition rate was one frame per second in the normal resolution mode. The duration of recording was 1 min. Blood samples were collected from the abdominal aorta for blood biochemical analysis after the liver surface scan. Finally, the liver, perirenal fat, and testicular fat were collected, weighed, and stored at -80°C for further experiments.