Mouse monoclonal anti nr2b

Mouse monoclonal anti-NFκB p65, mouse monoclonal anti-CaMKII, rabbit polyclonal anti-BDNF, and rabbit polyclonal anti-integrin αM antibodies were purchased from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA, USA). Mouse monoclonal anti-actin, mouse monoclonal anti-NR2B and rabbit polyclonal anti-NR2A antibodies were purchased from Millipore (Billerica, MA, USA). Mouse monoclonal anti-GFAP, rabbit monoclonal anti-phospho-NFκB p65, rabbit monoclonal anti-TNF-α, rabbit monoclonal anti-IL-6, mouse monoclonal anti-IL-1β, and horseradish peroxidase (HRP)-conjugated IgG antibodies were purchased from Cell Signaling Technology, Inc. (Danvers, MA, USA). ECL Western Blotting Reagents were purchased from GE Healthcare (Little Chalfont, Buckinghamshire, UK). Other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Ro25-6981 hydrochloride hydrate was purchased from Sigma-Aldrich (Millipore Sigma, USA), and was administered 30 min (min) before behavioral tests. The following primary antibodies and dilutions were used for immunohistochemistry (IHC) staining: rat anti-BrdU IgG (1:1,000; Abcam), mouse monoclonal anti-GFAP (1:1,000; Millipore), mouse monoclonal anti-NeuN (1:200; Millipore), anti-rat FITC (1:200; Invitrogen, Carlsbad, CA), anti-mouse Rhodamine Red-X (1:200; Invitrogen), and anti-rabbit Cy5 (1:200; Invitrogen). The antibodies below were used for immunoblotting: mouse monoclonal anti-NR2B (1:1,000; Millipore), mouse monoclonal anti-BDNF (1:1,000; Millipore), rabbit monoclonal anti-phospho CREB and anti-CREB (both 1:1,000; Millipore) and β-actin (1:10,000; Abcam). Alexa Fluor 700 conjugated goat anti-rabbit or goat anti-mouse antibodies (both 1:20,000; Invitrogen, Eugene, OR) were used as secondary antibodies.