Fitc conjugated anti cd206

Manufactured by BioLegend

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FITC-conjugated anti-CD206 is a monoclonal antibody that recognizes the CD206 antigen, also known as the macrophage mannose receptor. This antibody is conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate), which allows for the detection and analysis of CD206-expressing cells using flow cytometry or other fluorescence-based techniques.

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CD206 (177 citations) Anti-CD206 (52 citations) CD206-FITC (49 citations) Anti-CD206-FITC (13 citations) FITC-conjugated anti-mouse CD206 (8 citations) FITC-conjugated anti-CD206 antibody (4 citations) FITC-conjugated CD206 (2 citations) FITC-conjugated anti-mouse CD206 antibody (2 citations)

6 protocols using fitc conjugated anti cd206

Characterizing Lung Immune Cells

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After blocking the Fc receptors, isolated BALF lung cells were washed and
resuspended in PBS supplemented with 1% fetal bovine serum. Cells were stained
with FITC-conjugated anti-CD206 (BioLegend, San Diego, CA, USA), PE-conjugated
anti-CD11b, and PE-conjugated anti-major histocompatibility complex (MHC) class
II (BD Biosciences, San Jose, CA, USA) for 30 minutes at 4°C.^{10 (link),11} Flow
cytometry was then performed using a Navious flow cytometer (Beckman Coulter,
Miami, FL, USA) for quantification.

Jo W.S., Kang S., Jeong S.K., Bae M.J., Lee C.G., Son Y., Lee H.J., Jeong M.H., Kim S.H., Moon C., Shin I.S, & Kim J.S. (2022). Low Dose Rate Radiation Regulates M2-like Macrophages in an Allergic Airway Inflammation Mouse Model. Dose-Response, 20(3), 15593258221117349.

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Multiparametric Immune Cell Profiling

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Single-cell suspensions were preincubated with FcγR-specific blocking mAb (2.4G2) and washed before staining. Cells were stained with the following antibodies Percp-cy5-conjugated anti-CD45 (eBioscience), APC-conjugated anti-F4/80 (eBioscience), PE-cy7-conjugated conjugated anti-CD11c (eBioscience), FITC–conjugated anti-CD206 (BioLegend), APC-conjugated anti-CD3 (eBioscience), FITC–conjugated anti-CD4 (eBioscience); For intracellular staining, cells were permeabilized (Cytofix/Cytoperm kit; BD Biosciences) and incubated with PE–conjugated anti-IL-4, PE–conjugated anti-IL-5, PE–conjugated anti-IFN-γ (eBioscience), rabbit anti-Ym-1 (Stem Cell Technologies) and PE–conjugated anti-rabbit IgG (eBioscience). Cells were analyzed on a LSRII (BD Biosciences) with FlowJo ver.10 software (TreeStar).

Lee H.S., Kwon H.S., Park D.E., Woo Y.D., Kim H.Y., Kim H.R., Cho S.H., Min K.U., Kang H.R, & Chang Y.S. (2015). Thalidomide Inhibits Alternative Activation of Macrophages In Vivo and In Vitro: A Potential Mechanism of Anti-Asthmatic Effect of Thalidomide. PLoS ONE, 10(4), e0123094.

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Macrophage Polarization Evaluation

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LPS was applied to induce macrophage polarization, while CD80 and CD206 were chosen to mark the M1 and M2 phenotypes, respectively. APC‐conjugated anti‐CD80 (104 714, Biolegend) and FITC‐conjugated anti‐CD206 (141 703, Biolegend) were used to evaluate macrophage subsets. RAW264.7 macrophages were planted in a six‐well plate at a density of 1 × 10⁶ cells per well overnight to allow attachment, then the cells were preincubated with MTem/Los (Tem: 600 ng mL⁻¹, Los: 50 µg mL⁻¹ at a final concentration) for 1 h and then stimulated with LPS (1 µg mL⁻¹) for 24 h. After collection, samples were treated according to the manufacturer's protocol and further analyzed via a flow cytometer.

Li S., Lu Z., Huang Y., Wang Y., Jin Q., Shentu X., Ye J., Ji J., Yao K, & Han H. (2022). Anti‐Oxidative and Anti‐Inflammatory Micelles: Break the Dry Eye Vicious Cycle. Advanced Science, 9(17), 2200435.

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Quantifying M1/M2 Macrophage Markers and Neuronal Apoptosis

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The percentage of M1-related CD16/32 positive cells and M2-related CD206 positive cells were examined by flow cytometry. Briefly, the BV-2 cells were collected, washed with PBS and adjusted to 1 × 10⁶ cells/mL. Afterward, PE-conjugated anti-CD16/32 (BioLegend, USA) and FITC-conjugated anti-CD206 (BioLegend) were added. After 1 h of incubation at 4 °C in the dark, the cells were washed with PBS and resuspended in 500 μL of PBS solution. The mixtures were analyzed with a BD Accuri™ C6 flow cytometer (BD Biosciences, USA).
To evaluate cell apoptosis in the primary mouse hippocampal neurons, flow cytometry analysis was performed using the FITC-conjugated Annexin V apoptosis detection kit (BD Pharmingen, USA) according to the manufacturer’s instructions. The data were analyzed using FlowJo software (Tree Star, USA).

Liu L., Xu Y., Dai H., Tan S., Mao X, & Chen Z. (2020). Dynorphin activation of kappa opioid receptor promotes microglial polarization toward M2 phenotype via TLR4/NF-κB pathway. Cell & Bioscience, 10, 42.

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Immune Cell Profiling of Oral Mucosa

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For analysis of immune infiltrate, oral mucosal tissues were harvested and digested in 1mg/ml collagenase I (Sigma) for 45 minutes at 37°C. The digested palate mucosa was filtered through a cell strainer to obtain a single cell suspension. Single-cell suspensions from palatal samples were stained for live cells using either Zombie Green or Zombie NIR (Biolegend) dyes in cell-culture grade PBS per manufacturer instructions. Cells were then stained with cell phenotyping antibodies in a 1:1 volume ratio of 3% FBS and Brilliant Stain Buffer (BD Biosciences) according to standard procedures and analyzed on a FACS AriaIIIu flow cytometer (BD Biosciences). The following antibodies were used for cell phenotyping: Zombie NIR Fixable Viability Kit (BioLegend), BV421-conjugated anti-CD11b (BioLegend), BV510-conjugated anti-Ly6C (BioLegend), BV711-conjugated anti-CD64 (BioLegend), APC anti-mouse/PE-conjugated anti-MerTK (Biolegend), FITC-conjugated anti-CD206 (BioLegend), 30μL of Accucheck Counting Beads (Invitrogen) were added per sample for absolute quantification of cell populations.

Ballestas S.A., Turner T., Kamalakar A., Stephenson Y., Willett N., Goudy S.L, & Botchwey E.A. (2019). Improving Hard Palate Wound Healing Using Immune Modulatory Autotherapies. Acta biomaterialia, 91, 209-219.

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Isolation and Characterization of Immune Cells

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Freshly obtained pancreases were rinsed in PBS, and then minced into little pieces. Tissues were digested in Hank’s Balanced Salt Solutions (HBSS) (TBD, Tianjin, China) with 2 mg/ml V collagenase (Sigma-Aldrich) for 10–15 min at 37 ℃ with shaking. D-HBSS (TBD, Tianjin, China) was added to terminate the digestion and the cell suspensions were filtered through a 400-mesh metal filter and centrifuged at 1500 rpm for 5 min. The pellets were then incubated with PE-conjugated anti F4/80 (eBioscience), FITC-conjugated anti-CD206 (BioLegend, San Diego, CA, USA Clone: C068C2) and APC-conjugated anti-CD11c (Miltenyi Biotec, Bergisch Gladbach, Germany) antibodies for flow cytometry analysis.
Freshly harvested BMDM were washed twice and then stained with PE-conjugated anti F4/80 (eBioscience) antibody for flow cytometry analysis. The THP-1 cells were washed twice before incubated with fix&perm and perm (BD), and them stained with PE-conjugated anti CXCL-10 (eBioscience) antibody for flow cytometry analysis.

Yin Y., Hao H., Cheng Y., Zang L., Liu J., Gao J., Xue J., Xie Z., Zhang Q., Han W, & Mu Y. (2018). Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice. Cell Death & Disease, 9(7), 760.

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