All samples were centrifuged for 10 min at 14,000 rpm using an Eppendorf Centrifuge 5804 (Vaudaux-Eppendorf AG, Schönenbuch, Switzerland), before being analyzed using a UHPLC–MS/MS system consisting of a Waters ACQUITY UPLC® core system and a Waters XEVO™ TQ-S micro tandem quadrupole mass spectrometer (Milford, MA, USA). An ACQUITY UPLC®BEH C18 column, 1.7 µm, 25 × 2.1 mm connected to an ACQUITY UPLC®BEH C18 Vanguard™ pre-column, 1.7 µm, 5 × 2.1 mm was used for chromatographic separation. Mass spectrometry was performed in multiple reaction monitoring (MRM) mode. All details of the analytical methods used for each of the molecules tested can be found in the Supplementary Information .
Xevo tq s micro tandem quadrupole mass spectrometer
Manufactured by Waters Corporation
Sourced in United States
The Xevo TQ-S micro tandem quadrupole mass spectrometer is a high-performance analytical instrument designed for sensitive and precise quantitative analysis. It utilizes tandem quadrupole technology to provide accurate mass detection and precise quantification of target analytes in complex samples.
Automatically generated - may contain errors
Lab products found in correlation
Quanlynx software, by Waters Corporation (2 mentions)
Acquity uplc core system, by Waters Corporation (1 mentions)
Uplc beh c18 1.7 m column, by Waters Corporation (1 mentions)
Agilent 7890b gas chromatograph, by Agilent Technologies (1 mentions)
Rxi 5ms capillary column, by Restek (1 mentions)
Beh c18 column, by Waters Corporation (1 mentions)
Acquity uplc system, by Waters Corporation (1 mentions)
6 protocols using xevo tq s micro tandem quadrupole mass spectrometer
1
UHPLC-MS/MS Analysis of Biomolecules
2
Quantification of Darunavir by LC-MS/MS
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DRV intracellular concentrations were determined using an adapted version of a previously published liquid chromatography-tandem mass spectrometry (LC–MS/MS) method17 (link). Cell suspensions were vortex mixed, sonicated for 5 min, placed on an orbital shaker for 2 h and centrifuged for 10 min at 10,500×g. The supernatant was transferred to a vial for injection and the pellet was set aside for protein quantification. Calibrators ranging from 1.25 to 125 ng/ml were prepared in a similar fashion. Chromatographic separation was achieved on a Waters UPLC BEH C18 1.7 µm column (2.1 × 50 mm) maintained at 40 °C. The injection volume was 5 µl and the flow rate was 0.5 ml/min. The mobile phase consisted of a gradient of water/formic acid 0.1% (mobile phase A) and acetonitrile (mobile phase B), starting with 95% A and 5% B, ramping up to 80% B over 6 min, then returning to 5% B at 6.1 min and remaining at this ratio until the end of the run (total run time: 8 min). The MS system was a Xevo TQS-micro tandem quadrupole mass spectrometer (Waters). The following ion transitions were monitored: 548.2 > 392.3 for DRV and 557.3 > 113 for DRV-d9.
3
GC-MS Analysis of Compounds
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The analysis was done using an Agilent® 7890B gas chromatograph (Agilent Technologies Inc., Santa Clara, CA, USA) coupled to a Waters™ Xevo™ TQ-S micro tandem quadrupole mass spectrometer (Waters Corporation, Milford, MA, USA), fitted with an APGC source, operated in positive ion mode, and controlled by MassLynx™ 4.2 software. A Restek™ Rxi™-5ms capillary column (30 m × 0.25 mm × 0.25 µm, Restek Corporation, Bellefonte, PA, USA) was used for GC separation. The oven temperature was set at 60 °C (2 min hold) initially, increased to 320 °C at 25 °C/min, and finally maintained at 320 °C for 5 min. Helium (99.999% purity) was used as a carrier gas in a constant flow of 1.2 mL/min and the injection volume was 1 µL with an autosampler in the split ratio of 1:10. The mass spectrometer parameters were set as follows: corona current at 0.4 µA, cone voltage at 0 V, cone gas (N2) at 20 L/h under wet condition and 90 L/h under dry condition, auxiliary gas (N2) at 250 L/h, and make-up gas (N2) at 275 mL/min. The initial instrument setup provides dry conditions, whereas wet conditions are obtained by adding water to the source region using a vial in a holding tray placed in the source enclosure.
4
In Vitro Inhibition of CYP3A7 by PFOS
5
Quantification of Protease Inhibitors by UPLC-MS/MS
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The Waters
Acquity UPLC system interfaced by electrospray ionization with a Waters
Quattro Premier XE triple quadrupole (or a Xevo TQ-S micro tandem
quadrupole) mass spectrometer (Waters Corp., Milford, MA) was used
in the positive ionization mode and with the MRM scan type. The following
source conditions were applied: 1.5 (or 1) kV for the capillary voltage,
120 °C for the source temperature, 450 °C for the desolvation
temperature, 50 L/h for the cone gas flow, and 600 (or 900) L/h for
the desolvation gas flow. The following mass transitions, CEs, and
CVs were used to detect the respective analytes: 629.5 > 183 (CE
=
22 V; CV = 30 V) for lopinavir, 721.5 > 296 (CE = 20 V; CV = 35
V)
for ritonavir, and 672 > 570 (CE = 32 V; CV = 40 V) for saquinavir,
used as the internal standard. Protease inhibitors were separated
on a Waters BEH C18 column (1.7 μm, 2.1 × 100 mm) by flowing
0.1% formic acid in water and acetonitrile at 0.5 mL/min. The following
gradient was used: 30% organic (acetonitrile) held for 0.5 min, increased
to 98% over 3.5 min, and held at 98% for 1.4 min. The MS peaks were
integrated using QuanLynx software (version 4.1, Waters Corp., Milford,
MA), and the analyte/internal standard peak area ratios were used
for further calculations.
Acquity UPLC system interfaced by electrospray ionization with a Waters
Quattro Premier XE triple quadrupole (or a Xevo TQ-S micro tandem
quadrupole) mass spectrometer (Waters Corp., Milford, MA) was used
in the positive ionization mode and with the MRM scan type. The following
source conditions were applied: 1.5 (or 1) kV for the capillary voltage,
120 °C for the source temperature, 450 °C for the desolvation
temperature, 50 L/h for the cone gas flow, and 600 (or 900) L/h for
the desolvation gas flow. The following mass transitions, CEs, and
CVs were used to detect the respective analytes: 629.5 > 183 (CE
=
22 V; CV = 30 V) for lopinavir, 721.5 > 296 (CE = 20 V; CV = 35
V)
for ritonavir, and 672 > 570 (CE = 32 V; CV = 40 V) for saquinavir,
used as the internal standard. Protease inhibitors were separated
on a Waters BEH C18 column (1.7 μm, 2.1 × 100 mm) by flowing
0.1% formic acid in water and acetonitrile at 0.5 mL/min. The following
gradient was used: 30% organic (acetonitrile) held for 0.5 min, increased
to 98% over 3.5 min, and held at 98% for 1.4 min. The MS peaks were
integrated using QuanLynx software (version 4.1, Waters Corp., Milford,
MA), and the analyte/internal standard peak area ratios were used
for further calculations.
6
Quantitative uHPLC-MS/MS Analysis of Metarrestin
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