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Mir 23a

Manufactured by GenePharma
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MiR-23a is a laboratory equipment product offered by GenePharma. It is a microRNA (miRNA) that plays a role in gene expression regulation. The core function of MiR-23a is to serve as a tool for studying gene regulation in biological research.

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Lab products found in correlation

Lipofectamine rnaimax, by Thermo Fisher Scientific (2 mentions) Mir control, by GenePharma (1 mentions) Pcdna gas5, by GenePharma (1 mentions) Pcdna gas5, by Thermo Fisher Scientific (1 mentions) Sirna control si control, by GenePharma (1 mentions) Pcdna control, by GenePharma (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Si gas5, by GenePharma (1 mentions)

Spelling variants of this product

MiR-23a mimics (5 citations) MiR-23a-3p inhibitor (3 citations) MiR-23a-3p mimic (3 citations) MiR‐23a inhibitor (2 citations)

2 protocols using mir 23a

1

Regulation of GAS5 by miR-23a in Lung Cancer

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miR-23a mimics (miR-23a), control mimics (miR-control), siRNA against GAS5 (si-GAS5), siRNA control (si-control), pcDNA-GAS5, and pcDNA empty control (pcDNA-control) were purchased from GenePharma (Shanghai, P.R. China). Cultured A549 and H157 cells were transfected with miR-23a, si-GAS5, pcDNA-GAS5, pcDNA-GAS5 + miR-23a, or their corresponding control at approximately 60%–70% confluence using Lipofectamine 2000 (Invitrogen). Transfection efficiency was checked by quantitative real-time polymerase chain reaction (qRT-PCR). Subsequent experiments were performed 48 h after transfection.
Mei Y., Si J., Wang Y., Huang Z., Zhu H., Feng S., Wu X, & Wu L. (2017). Long Noncoding RNA GAS5 Suppresses Tumorigenesis by Inhibiting miR-23a Expression in Non-Small Cell Lung Cancer. Oncology Research, 25(6), 1027-1037.
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2

Transfection of Small RNAs

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Small nucleotides such as miR-23a, anti-miR-23a, and their negative controls (NCs) were purchased from GenePharma (Shanghai, People’s Republic of China). The small interfering RNAs (siRNAs) for p53 and FOXA1 were commercially available from Santa Cruz Biotechnology Inc. (Dallas, TX, USA). Transfections were performed using Lipofectamine RNAiMAX (Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol provided by the manufacturer.
Sun Y., Tao C., Huang X., He H., Shi H., Zhang Q, & Wu H. (2016). Metformin induces apoptosis of human hepatocellular carcinoma HepG2 cells by activating an AMPK/p53/miR-23a/FOXA1 pathway. OncoTargets and therapy, 9, 2845-2853.
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