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Irdye 800cw donkey anti rabbit igg 926 32213

Manufactured by LI COR

IRDye 800CW donkey anti-rabbit-IgG (926-32213) is a secondary antibody conjugated with the IRDye 800CW fluorescent dye. It is designed to detect and quantify rabbit primary antibodies in various immunoassay applications.

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2 protocols using irdye 800cw donkey anti rabbit igg 926 32213

1

Western Blot Antibody Validation Procedure

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Mouse IFN1 polyclonal antibody (Ab) was prepared and conserved by our lab. Anti-IRF3 rabbit polyclonal antiserum was previously prepared and presented by Yibing Zhang, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China (25 (link)). These Abs were tested by Western blotting before experiments. The VP56 Ab was produced and conserved in our lab. Hemagglutinin (HA) tag (ab18181) mouse monoclonal Ab, Flag tag (ab125243) mouse monoclonal Ab, and β-tubulin rabbit polyclonal Ab (ab6046) were purchased from Abcam. GFP mouse monoclonal Ab (AE012) and GST mouse monoclonal Ab (AE001) were purchased from Abclonal. IRDye 800CW donkey anti-rabbit-IgG (926-32213) and anti-mouse-IgG(H+L) (926-32212) secondary Abs were purchased from LI-COR. Goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary Ab (A0216) was purchased from Beyotime.
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2

Western Blot Antibody Validation Procedure

Check if the same lab product or an alternative is used in the 5 most similar protocols
Mouse IFN1 polyclonal antibody (Ab) was prepared and conserved by our lab. Anti-IRF3 rabbit polyclonal antiserum was previously prepared and presented by Yibing Zhang, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China (25 (link)). These Abs were tested by Western blotting before experiments. The VP56 Ab was produced and conserved in our lab. Hemagglutinin (HA) tag (ab18181) mouse monoclonal Ab, Flag tag (ab125243) mouse monoclonal Ab, and β-tubulin rabbit polyclonal Ab (ab6046) were purchased from Abcam. GFP mouse monoclonal Ab (AE012) and GST mouse monoclonal Ab (AE001) were purchased from Abclonal. IRDye 800CW donkey anti-rabbit-IgG (926-32213) and anti-mouse-IgG(H+L) (926-32212) secondary Abs were purchased from LI-COR. Goat anti-mouse IgG horseradish peroxidase (HRP)-conjugated secondary Ab (A0216) was purchased from Beyotime.
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