Rac1 CDS-targeting- and Bcl2l1 CDS-targeting shRNA in lentiviral plasmid (TRCN0000310888 and TRCN0000004685) and control shRNA (SHC216V) were purchased from Sigma Aldrich. For viral production, 293T cells were transfected with lentiviral gag/pol and VSV-G (courtesy of Donald Kohn, UCLA) and the lentiviral plasmids, at a ratio of 2.2: 1.2: 3.3 (in [μg], gag/pol:VSVG:Plasmid) using Lipofectamine 3000 and P300 Enhancer. Viral particles were collected after 24 h and 48 h. One milliliter of viral supernatant was used to infect 0.75 × 106 BM lin− cells. Infected cells were collected the next day and irradiated with 300 cGy followed by treatment with or without 1 μg/mL DJ001, prior to CFC assay.
Shc216v
Manufactured by Merck Group
The SHC216V is a laboratory equipment product offered by Merck Group. It serves as a centrifuge, a device used to separate components of a liquid mixture based on their relative densities. The SHC216V model provides a core centrifugation function without further details on its intended use or applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using shc216v
1
Rac1 and Bcl2l1 Knockdown in BM Lin- Cells
2
Lentiviral Knockdown of LIN28B
3
Lentiviral Knockdown of LIN28B
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LIN28B shRNA in lentiviral plasmid (TRCN0000122191 and TRCN0000122599) and control shRNA (SHC216V) were purchased from Sigma-Aldrich. For viral production, 293T cells were transfected with lentiviral gag/pol, VSV-G, and the lentiviral plasmid, at a ratio of 1:0.4:1, using Lipofectamine 2000. Viral particles were harvested after 24hrs and 48hrs. Two milliliters of viral supernatant were used to infect 1,000,000 cells in the presence of Polybrene (8 ng/µL). Infected cells were selected on Puromycin antibiotic before subsequent analysis.
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