Mice were treated with systemic thermal therapy (STT; core temperature of 39.5 ± 0.5°C, 6 h) and allowed to return to baseline temperature prior to homing studies as described14 (link),27 (link). LPS (1 mg/kg; Sigma) was injected i.p. 6 h prior to trafficking studies14 (link). Neutralizing polyclonal anti-CXCL9 or anti-CXCL10 Ab30 (link) (100 μg/mouse) or polyclonal isotype IgG control (cat# BE0091 100 μg/ mouse; BioXCell) were injected i.p. 3 h prior to competitive homing studies. Murine activated CD8+ T cells were pretreated with pertussis toxin (100 ng/ml, 2.5 h; Sigma), or anti-mouse CXCR3 blocking Ab (cat# 126526 100 μg/mL, 1h; Biolegend). Human activated T cells were pretreated with pertussis toxin, human CXCR3 functional blocking Ab (cat# LS-C6283-500 40 μg/ml, 30 min; LifeSpan BioSciences, Inc39 (link)), or human recombinant CXCL10 (500nM, 30 m; Peprotech) to desensitize CXCR340 (link).
Polyclonal isotype igg control
Manufactured by BioXCell
Polyclonal isotype IgG control is a laboratory reagent used as a control in immunoassays. It consists of a mixture of immunoglobulin G (IgG) antibodies from multiple sources, which serve as a reference to compare the specificity and activity of target antibodies.
Automatically generated - may contain errors
2 protocols using polyclonal isotype igg control
1
Investigating Immune Cell Trafficking Regulation
2
Investigating Immune Cell Trafficking Regulation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Mice were treated with systemic thermal therapy (STT; core temperature of 39.5 ± 0.5°C, 6 h) and allowed to return to baseline temperature prior to homing studies as described14 (link),27 (link). LPS (1 mg/kg; Sigma) was injected i.p. 6 h prior to trafficking studies14 (link). Neutralizing polyclonal anti-CXCL9 or anti-CXCL10 Ab30 (link) (100 μg/mouse) or polyclonal isotype IgG control (cat# BE0091 100 μg/ mouse; BioXCell) were injected i.p. 3 h prior to competitive homing studies. Murine activated CD8+ T cells were pretreated with pertussis toxin (100 ng/ml, 2.5 h; Sigma), or anti-mouse CXCR3 blocking Ab (cat# 126526 100 μg/mL, 1h; Biolegend). Human activated T cells were pretreated with pertussis toxin, human CXCR3 functional blocking Ab (cat# LS-C6283-500 40 μg/ml, 30 min; LifeSpan BioSciences, Inc39 (link)), or human recombinant CXCL10 (500nM, 30 m; Peprotech) to desensitize CXCR340 (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!