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Anti ps744 748 pkd antibody

Manufactured by Abcam
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Anti-pS744/748-PKD antibody is a lab equipment product that can be used to detect the phosphorylation of serine residues 744 and 748 in the protein kinase D (PKD) enzyme. This antibody can be utilized in various analytical techniques to study the activation and signaling pathways of PKD.

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Lab products found in correlation

Envision plus dual labeled polymer kit, by Agilent Technologies (2 mentions) Sodium citrate buffer ph 6, by Agilent Technologies (2 mentions) Protein block serum free solution, by Agilent Technologies (2 mentions) Antibody diluent background reducing solution, by Agilent Technologies (2 mentions)

2 protocols using anti ps744 748 pkd antibody

1

Immunohistochemical Analysis of PKD Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Slides were de-paraffinized (1 hr, 60° C), de-waxed in xylene (5 times, 4 min) and gradually re-hydrated with ethanol (100%, 95%, 75%, each 2 times, 3 min). Re-hydrated samples were rinsed in water and subjected to antigen retrieval in 10 mM sodium citrate buffer pH 6.0 (DAKO, Carpinteria, CA). Slides were treated with 3% H2O2 (5 min) to reduce endogenous peroxidase activity, washed with PBS containing 0.5% Tween 20, and blocked with protein block serum free solution (DAKO) for 5 min at RT. Samples were stained with H&E, alcian blue, anti-claudin-18 antibody (1:1000), anti-PKD1 antibody (1:100), anti-PKD2 antibody (1:200), anti-PKD3 antibody (1:200), or anti-pS744/748-PKD antibody (Abcam) at a dilution of 1:50 in Antibody Diluent Background Reducing Solution (DAKO) and visualized using the Envision Plus Dual Labeled Polymer Kit (DAKO) according to the manufacturer’s instructions. Images were captured using the ScanScope XT scanner and ImageScope software (Aperio, Vista, CA).
Liou G.Y., Döppler H., Braun U.B., Panayiotou R., Buzhardt M.S., Radisky D.C., Crawford H.C., Fields A.P., Murray N.R., Wang Q.J., Leitges M, & Storz P. (2015). Protein kinase D1 drives pancreatic acinar cell reprogramming and progression to intraepithelial neoplasia. Nature communications, 6, 6200.
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2

Immunohistochemical Analysis of PKD Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Slides were de-paraffinized (1 hr, 60° C), de-waxed in xylene (5 times, 4 min) and gradually re-hydrated with ethanol (100%, 95%, 75%, each 2 times, 3 min). Re-hydrated samples were rinsed in water and subjected to antigen retrieval in 10 mM sodium citrate buffer pH 6.0 (DAKO, Carpinteria, CA). Slides were treated with 3% H2O2 (5 min) to reduce endogenous peroxidase activity, washed with PBS containing 0.5% Tween 20, and blocked with protein block serum free solution (DAKO) for 5 min at RT. Samples were stained with H&E, alcian blue, anti-claudin-18 antibody (1:1000), anti-PKD1 antibody (1:100), anti-PKD2 antibody (1:200), anti-PKD3 antibody (1:200), or anti-pS744/748-PKD antibody (Abcam) at a dilution of 1:50 in Antibody Diluent Background Reducing Solution (DAKO) and visualized using the Envision Plus Dual Labeled Polymer Kit (DAKO) according to the manufacturer’s instructions. Images were captured using the ScanScope XT scanner and ImageScope software (Aperio, Vista, CA).
Liou G.Y., Döppler H., Braun U.B., Panayiotou R., Buzhardt M.S., Radisky D.C., Crawford H.C., Fields A.P., Murray N.R., Wang Q.J., Leitges M, & Storz P. (2015). Protein kinase D1 drives pancreatic acinar cell reprogramming and progression to intraepithelial neoplasia. Nature communications, 6, 6200.
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