Human 2.0 st array

Manufactured by Thermo Fisher Scientific

The Human 2.0 ST array is a microarray product designed for analyzing gene expression. It provides a comprehensive view of the human transcriptome by covering protein-coding and non-coding RNA features. The array enables researchers to study various aspects of human biology and disease.

Automatically generated - may contain errors

Lab products found in correlation

Rneasy mini kit, by Qiagen (1 mentions) Human genome u133 plus 2.0 array, by Thermo Fisher Scientific (1 mentions) Transcriptome analysis console, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

GeneChip® Human Gene 2.0 ST oligonucleotide arrays (2 citations) GeneChip® Human Gene 2.0 ST whole-transcript arrays (2 citations) Human Gene 1.0 ST and 2.0 ST arrays (2 citations) Arrays of human Hugene-2_0-st-type (2 citations) Human Gene 2.0 ST GeneChip® arrays (2 citations)

2 protocols using human 2.0 st array

Microarray Analysis of MCF-7 Cancer Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

We downloaded raw CEL files generated using the Affymetrix Human Genome U133 Plus 2.0 Array (GPL570) platform from the public microarray database Gene Expression Omnibus, and normalized them using the Universal exPression Code method (SCAN.UPC package of Bioconductor)30 (link). Samples were grouped by normal and cancer according to the annotations in the original GSM files. For microarray experiment, total RNAs were isolated from MCF-7 cells using an RNeasy Mini Kit (Qiagen, Hilden, Germany), and delivered to commercial microarray service (eBiogen, Seoul, Republic of Korea). The assay was performed using Affymetrix human 2.0 ST array.

Park J., Lee J, & Choi C. (2015). Evaluation of drug-targetable genes by defining modes of abnormality in gene expression. Scientific Reports, 5, 13576.

+ Open protocol

+ Expand

Transcriptome Analysis of Oligodendroglioma

Check if the same lab product or an alternative is used in the 5 most similar protocols

The mRNA expression profiles of 13 ODGs (6 Grade II, 7 Grade III) and 3 normal brains were examined using Affymetrix Human 2.0 ST array (Affymetrix Inc, CA) as per the protocols outlined in detail in the geneChip@ expression analysis technical manual (Affymetrix, http://www.affymetrix.com/support/downloads/manuals/expression_analysis_technical_manual.pdf). mRNA signal intensities were log2 transformed and analyzed for differentially expressed mRNAs using the Transcriptome Analysis Console (TAC) (Affymetrix Inc, CA) [22 (link)]. Differentially detected mRNA signals with ≥ 2.0 fold-change and the P < 0.05 were considered significant.

Kumar A., Nayak S., Pathak P., Purkait S., Malgulawar P.B., Sharma M.C., Suri V., Mukhopadhyay A., Suri A, & Sarkar C. (2018). Identification of miR-379/miR-656 (C14MC) cluster downregulation and associated epigenetic and transcription regulatory mechanism in oligodendrogliomas. Journal of Neuro-Oncology, 139(1), 23-31.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!