3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt dimethyl sulfoxide dmso

S180 cells (murine sarcoma cells) were bought from the Shanghai Institute of Biological Sciences at the Chinese Academy of Sciences (Shanghai, China). Bovine serum albumin (BSA), standard monosaccharides, glucuronic acid, trifluoroacetic acid (TFA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), dimethyl sulfoxide (DMSO), and 5-fluorouracil (5-FU) were obtained from Solarbio (Shanghai, China). Pectinex BE XXL (16,000 PGU/mL) were obtained from Novozymes biotechnology Co., Ltd. (Tianjin, China). Medium RPMI-1640 was provided by Hyclone (Thermo Scientific Inc., Waltham, MA, USA). Fetal bovine serum (FBS) was purchased from Hangzhou Sijiqing Corp (Hangzhou, China). Cytokine detecting ELISA kits were bought from Lufeng Bio-Technique Co., Ltd. (Shanghai, China). PIPA, Bradford Protein Assay Kit, Enhanced chemiluminescence (ECL) kit were obtained from Solarbio Science &Technology Co., Ltd. (Beijing, China). The antibodies specific to β-actin, Bax and Bcl-2 were provided by Tianjin Sungene Biotech Co. (Tianjin, China). All other chemicals and agents were of analytical grades.

Chitosan (CS) with an average molecular weight of 6.5 kDa was supplied by Nantong Feiyu Biological Technology Co., Ltd. (Jiangsu, China). Curcumin (Cur, 95.0% purity) was purchased from Adamas-beta (Shanghai, China). Copper chloride (CuCl₂·2H₂O), 3 (N-morpholino)-propanesulfonic acid (MOPS) and acetic acid (99.0%) were obtained from Aladdin (Shanghai, China). Sodium hydroxide (NaOH, 99.0%) was obtained from J&K Chemicals. Ethanol and Tween 80 were purchased from Greagent. Ltd. (Shanghai, China). RPMI 1640 medium, fetal bovine serum (FBS), trypsin-EDTA, PBS, 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), dimethyl sulfoxide (DMSO) and human cancer cell H1299 were purchased from Solarbio science & technology Co., Ltd. (Beijing, China). All chemicals were of analytical grade. Deionized water was obtained from a Milli-Q water purification system and used in all experiments.