ALP is an early biomarker of osteoblast differentiation. After differentiation for 3 d, cells were washed twice with PBS, fixed with 10% formalin in PBS for 5 min, rinsed with deionized water, and stained using an ALP Kit (Sigma-Aldrich). To measure ALP activity, differentiated cells were washed twice with PBS, fixed with 10% formalin in PBS for 5 min, rinsed with PBS, and measured using a one-step PNPP substrate solution (Thermo Scientific, MA, USA) according to the manufacturer’s protocol.
One step pnpp substrate solution
Manufactured by Thermo Fisher Scientific
Sourced in United States
The One-step PNPP substrate solution is a ready-to-use liquid substrate for the colorimetric detection of enzyme-linked immunoassays. It contains p-nitrophenyl phosphate (PNPP) and buffer components in a single solution.
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Lab products found in correlation
2 protocols using one step pnpp substrate solution
1
Alkaline Phosphatase Activity Measurement
2
Alkaline Phosphatase Assay for Osteoblasts
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ALP is an early biomarker of osteoblast differentiation. After differentiation for 3 days, cells were washed twice with phosphate-buffered saline (PBS), fixed with 10% formalin in PBS for 5 minutes, rinsed with deionized water, and stained using an ALP Kit (Sigma-Aldrich). To measure ALP activity, differentiated cells were washed twice with PBS, fixed with 10% formalin in PBS for 5 minutes, rinsed with PBS, and measured using a one-step PNPP substrate solution (Thermo Scientific, MA) according to the manufacturer’s protocol.
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