Tenuazonic acid

Alternariol (AOH), Alternariol monomethyl ether (AME), altenuene (ALT), tenuazonic acid (TeA), N, N-dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), and gold chloride (HAuCl₄·3H₂O) were purchased from Sigma-Aldrich (Shanghai, China). Bovine serum albumin (BSA), human serum albumin (HSA), methyl 4-bromobutanoate, trisodium citrate dihydrate, and Tween-20 were purchased from Amresco (CochranSolon, OH, USA). PEG20000 was purchased from Alfa Aesar Chemical Co., Ltd. (Shanghai, China). Acetonitrile (HPLC grade) were purchased from Thermo Fisher Scientific (Waltham, NJ, USA). Incomplete Freund’s adjuvant, complete Freund’s adjuvant, 96-well microplates, goat anti-mouse IgG, and developing solution was offered by Beijing Kwinbon Biotechnology Co., Ltd. (Beijing, China). Polyvinyl chloride (PVC) sheet, NC membrane (Millipore 135), absorbent pad, conjugate pad, and sample pad were obtained from Shanghai Kinbio Tech. Co., Ltd. (Shanghai, China). Analytical grade sodium chloride (NaCl), dimethylformamide (DMF), methyl 4-bromobutanoate, potassium carbonate (K₂CO₃), ethanol, petroleum ether, tetrahydrofuran (THF), lithium hydroxide (LiOH), ethyl acetate (EtOAc), and other chemicals and solvents were ordered from Beijing Chemical Reagent Co. (Beijing, China). Water was deionized and purified with a Milli-Q system (Millipore, Bedford, MA, USA).

Tenuazonic acid and other common mycotoxin standards, bovine serum albumin (BSA) and goat anti-mouse IgG antibody (GAMA) were obtained from Sigma-Aldrich (MO, United States). Trisodium citrate, HAuCl₄ 4H₂O and hydroquinone were purchased from Macklin (Shanghai, China). Nitrocellulose (NC) membrane was provided by Jieyi BioTech. (Shanghai, China). Anti-TA antibody reagent (anti-TA McAb 6D5) was prepared in our laboratory according to our previous work (Cai et al., 2021 (link)). All the other reagents were analytical reagent grade. Ultraviolet-visible spectrum (UV-VIS) was tested by spectrophotometer (PerkinElmer, Waltham, MA, United States). Tecnai G2 F30 transmission electron microscope (TEM) (FEI, Hillsboro, OR, United States) was used to scan the diameter and shape of the prepared nanoparticles. The UHPLC-MS/MS was performed by Waters ACQUITY UPLC-XEVO TQ-S MS with ACQUITY UPLC BEH C18 (Waters, Milford, MA, United States).