Antibodies and reagents used in this study were as follows: anti-G3BP1 antibody (BD Bioscience, 611126, Franklin Lakes, NJ, USA), anti-PKR (Santa cruz biotechnology, sc-6282, Dallas, TX, USA) anti-RACK1 (Santa Cruz biotechnology sc-17754), anti-phospho-eIF2α (Ser51) (Cell Signaling Technology, 9721S, Danvers, MA, USA), anti-cleaved caspase-3 (Cell Signaling Technology, 9661S), anti-PARP (Cell Signaling Technology, 9542S), anti-Actin (Cell Signaling Technology, 3700S), anti-PKR (Abcam, ab52506, Cambridge, MA, USA), anti-phospho-PKR(Thr451) (Abcam, ab81303), anti-IMP1 antibody (Bethel Laboratories, A303-424A, Montgomery, TX, USA), anti-HA-HRP (Roche, 12013819001, Mannheim, Germany), morusin (root bark of Morus alba) (Biopurity Phytochemicals Ltd., BP0961, Chengdu, Sichuan, China), ISRIB (Millipore sigma, SML0843, Saint Louis, MO, USA), 3-methyladenine (Millipore sigma, M9281), zVAD-FMK (Santa cruz biotechnology, sc-3067).
Anti g3bp1 antibody
Manufactured by BD
Sourced in United States
The Anti-G3BP1 antibody is a laboratory reagent used in research applications. It is designed to detect and bind to the G3BP1 (Ras GTPase-activating protein-binding protein 1) protein, which is involved in the regulation of cellular stress response pathways. The antibody can be used in various immunoassay techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of G3BP1 in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Anti rack1, by Santa Cruz Biotechnology (1 mentions)
Anti pkr, by Santa Cruz Biotechnology (1 mentions)
Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions)
Anti phospho eif2α ser51, by Cell Signaling Technology (1 mentions)
Anti ha hrp, by Roche (1 mentions)
Z vad fmk, by Santa Cruz Biotechnology (1 mentions)
Anti pkr, by Abcam (1 mentions)
Anti actin, by Cell Signaling Technology (1 mentions)
Anti parp, by Cell Signaling Technology (1 mentions)
Isrib, by Merck Group (1 mentions)
3 methyladenine, by Merck Group (1 mentions)
Prolong gold antifade mountant with dapi, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Las x, by Leica (1 mentions)
Tcs sp8 confocal microscopy system, by Leica (1 mentions)
2 protocols using anti g3bp1 antibody
1
Antibodies and Reagents in Cell Study
2
Immunofluorescence Staining of G3BP1
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Cells were fixed with 4% paraformaldehyde in PBS for 15 min, permeabilized with 0.5% Triton X-100 in PBS for 10 min, and then blocked with 3% goat serum in PBST (PBS plus 0.1% Triton X-100) for 1 h, with all steps performed at RT. Cells were further incubated with anti–G3BP1 antibody (BD, 611127) overnight at 4 °C. AlexaFluor-488 (Invitrogen, A11001) used as the second antibody was incubated with the cells for 1 h at RT. After 3 washes with PBST, cells were mounted on glass slides using the ProLong Gold Antifade Mountant with DAPI (ThermoFisher, P36935). Images were captured using the Leica TCS SP8 confocal microscopy system with a ×100 objective (oil immersion). Images were processed and assembled into figures using LAS X (Leica) and Fiji.
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