Immobilion western cemiluminescent hrp substrate

Cell protein extraction, solubilization, and analysis by 1-D PAGE were performed as previously described [33 (link)]. The antibody against ABCG2 clone BXP-21 (diluition 1:1000) was from Abcam (Cambridge, UK). The antibody against GAPDH was from Ambion (Austin, TX). HRP-conjugated secondary antibodies were from Pierce (Rockford, IL) and the chemiluminescence system (ImmobilionTM Western Cemiluminescent HRP Substrate), was from Millipore (Temecula, CA).

Cell protein extraction, solubilization, and analysis by 1-D PAGE were performed as previously described [41 (link)]. Antibodies against HER2; p-HER2 ^Tyr1221/1222; p70S6K; p-p70S6K ^{Thr421/Ser424}; Akt; p-Akt ^Ser473; p44/42 MAPK; p-p44/42 MAPK; caspase-7 and 9; cyclin A and B1; Rb; p-Rb were from Cell Signaling Technology (Beverly, MA). Antibody against cytochrome-c (7H8) was form Santa Cruz Biotechnology Inc. (Dallas, TX). Antibodies against actin was from Sigma–Aldrich (St Louis, MO). Antibody against GAPDH was from Ambion (Austin, TX). HRP-conjugated secondary antibodies were from Pierce (Rockford, IL) and chemiluminescence system (ImmobilionTM Western Cemiluminescent HRP Substrate), was from Millipore (Temecula, CA).