Ab79414

Five monoclonal antibodies anti-HLA-DQA1 antibody (1:10,000 dilution for WB, 1:200 dilution for IHC, ab128959), anti-GZMA antibody (1:1000 dilution, ab209205), anti-CD79A antibody (1:10,000 dilution, ab79414), anti-LTB antibody (1:1000 dilution, ab89568) and anti-HE5 antibody (1:1000 dilution, ab125071) were purchased from Abcam (Cambridge, UK). Monoclonal antibody anti-β-actin (1:20,000 dilution, 66009-1-Ig) was purchased from Proteintech (Wuhan, China).

Formalin-fixed paraffin-embedded tissues were sectioned (4 μm) and stained with hematoxylin and eosin (H&E) for histological analysis or used for immunohistochemistry (IHC). For IHC, endogenous peroxidases were inactivated by 3% hydrogen peroxide and nonspecific signals were blocked by 1% BSA. Sections were incubated with the primary antibody: CD20 (1:200, #MA5-13141, Invitrogen), CD3 (1:200, #ab16669, Abcam), MECA-79 (1:100, #sc-19602, Santa Cruz), CD21 (1:100, #sc-13135, Santa Cruz), CD23 (1:100, #MA5-14572, Invitrogen), TCL1A (1:500, #ab108978, Abcam), PAX5 (1:1000, #ab109443, Abcam), TNFRSF13C (1:300, #ab168389, Abcam), CD79A (1:200, #ab79414, Abcam) at 4°C overnight, HRP-conjugated secondary antibody at 37°C for 1 h, and subsequently stained with DAB substrate. Counterstaining was performed with hematoxylin and mounted with a mounting medium.