Rub-4-AP can be uncaged with either 1 photon excitation (λ = 480 nm) or 2-photon excitation (800 nm) (Nikolenko et al., 2005 (link); Fino et al., 2009 (link)). Uncaging was performed with 470 nm LED light (Figure 1A , Thorlabs, Newton, NJ) either broadly to the entire cortex or focally, delivered through an optical fiber (OD: 200 μm, Thorlabs, Newton, NJ), using a high power LED driver (LEDD1B, Thorlabs, Newton, NJ), controlled by a stimulator (Master-8; AMPI, Jerusalem, Israel). Optical power delivered at the fiber tip was calibrated with a PM100USB power meter (Thorlabs, Newton, NJ).
Pm100usb power meter
Manufactured by Thorlabs
Sourced in United States, Sweden
The PM100USB is a compact and versatile power meter designed for measuring optical power. It features a USB interface for easy connection to a computer and supports a wide range of Thorlabs power sensor heads.
Automatically generated - may contain errors
3 protocols using pm100usb power meter
1
Uncaging Rub-4-AP for Neuronal Manipulation
2
Photoactivation Setup for Biological Experiments
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The light source used for the photoactivation process is a mounted blue LED at 405 nm (M405L4) [22 (link)]. The light beam was collimated using an aspheric condenser lens attached to the mounted LED using an SM1 Lens Tube to illuminate an area of 1 cm. A T-Cube LED Driver, 1200 mA Max Drive Current (LEDD1B) was used to control the output power. The output power was monitored using a PM100USB power meter connected to a S302C thermal sensor head, all purchased from Thorlabs (Newton, NJ, USA).
3
Spectroscopic Analysis of Urine and PpIX
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The absorption spectrum of urine was recorded using a UV-2700 double-beam spectrophotometer (Shimadzu, Holm & Halby, Denmark). The fluorescence spectra of PpIX and urine were measured using a Fluorolog 3 spectrofluorometer (Horiba Jobin Yvon, Longjumeau, France). PpIX samples prepared as described in section 2.1 were placed in a suitable fluorescence cuvette in the spectrofluorometer and measured in right-angle geometry. The spectral output of the high-power LED light source was measured using a QE65000 CCD spectrograph from Ocean Optics (Dunedin, FL, USA). The power density of the light source was measured using a PM100USB power meter fitted with a S120VC photodiode power sensor both from Thorlabs (Thorlabs AB, Gothenburg, Sweden). To prevent saturation of spectrometer and power meter, a reflective neutral density filter ND with an optical density of 4 was used from Thorlabs (Thorlabs AB, Gothenburg, Sweden).
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