Equal amounts of fresh and lyophilized leaf materials were extracted in the same volume of extraction buffer, and the extracted proteins were loaded in a serially diluted way and followed by immunoprobing steps described above. For lettuce lines expressing CTB-Pro-IGF-1 or marker-free PTD-Pro-IGF-1, 1X indicates the volume of 10 μLl from the resuspended protein samples, which were prepared from the extraction of 10 mg of lyophilized powder in 300 μL of protein extraction buffer. The sample preparation of tobacco line expressing CTB-Pro-IGF-1 was the same as the lettuce line but 1X indicates the volume of 1 μL. For loading controls, membranes were deprobed using Western Stripping Buffer (Thermo Fisher Scientific, Waltham, MA) for 10 min at 37 °C, then immunolabeled with rabbit anti-RbcL antibody (Rubisco Large Subunit) (Agrisera, Vannas, Sweden) diluted 1 in 40,000 in PTM for 1 h. The subsequent western blot steps were followed as described above.
Rabbit anti rbcl antibody rubisco large subunit
Manufactured by Agrisera
Sourced in Sweden
Rabbit anti-RbcL antibody (Rubisco Large Subunit) is a laboratory reagent used to detect and quantify the large subunit of the Rubisco enzyme, a key component of the photosynthetic process in plants. This antibody is produced in rabbits and can be used in various immunoassay techniques, such as Western blotting, ELISA, and immunohistochemistry, to identify and measure the presence of the Rubisco large subunit in biological samples.
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2 protocols using rabbit anti rbcl antibody rubisco large subunit
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Quantitative Immunoblotting of Recombinant Proteins
2
Quantitative Immunoblotting of Recombinant Proteins
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Equal amounts of fresh and lyophilized leaf materials were extracted in the same volume of extraction buffer, and the extracted proteins were loaded in a serially diluted way and followed by immunoprobing steps described above. For lettuce lines expressing CTB-Pro-IGF-1 or marker-free PTD-Pro-IGF-1, 1X indicates the volume of 10 μLl from the resuspended protein samples, which were prepared from the extraction of 10 mg of lyophilized powder in 300 μL of protein extraction buffer. The sample preparation of tobacco line expressing CTB-Pro-IGF-1 was the same as the lettuce line but 1X indicates the volume of 1 μL. For loading controls, membranes were deprobed using Western Stripping Buffer (Thermo Fisher Scientific, Waltham, MA) for 10 min at 37 °C, then immunolabeled with rabbit anti-RbcL antibody (Rubisco Large Subunit) (Agrisera, Vannas, Sweden) diluted 1 in 40,000 in PTM for 1 h. The subsequent western blot steps were followed as described above.
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