Biocoat growth factor reduced matrigel invasion chamber with 8.0 mm pet membrane in 24 well plate

Invasion across a basement membrane was performed using Corning Biocoat™ Growth Factor Reduced Matrigel Invasion Chamber with 8.0 mm PET membrane in 24-well plate (Corning Life Sciences, Corning, NY) according to manufacturer’s instructions. Briefly, a volume of 2.5 × 10⁴ cells in medium that were treated with indicated factors for 24 h were added to each invasion chamber. After 18–24 h, invaded cells located on the underside of chambers were fixed with formaldehyde for 10 min and stained with trypan blue before counting. Transwell migration assays were performed essentially as the invasion assays above except that a 8-μm Biocoat Cell Culture Control Inserts was used instead of the Matrigel inserts, and the migrating cells were counted after incubation of 1.0 × 10⁴ cells for 8 h.