Target cells (K562, H929, MM.1R, U266, RPMI8226) were plated into a 96-well U-bottom plate at a density of 2 × 104 cells/well and co-cultured with effector cells (Coc NK92, CAR NK92) at a ratio of 1:1 for 4 h at 37 °C. After 4 h of co-culturing, plates were centrifuged at 1000× g for 5 min and supernatants were harvested for ELISA. The concentration of IFN-γ in each well was determined using a human IFN-γ ELISA kit (#421203, Biolegend, San Diego, CA, USA). The concentration of granzyme B was determined using a human granzyme B ELISA kit (#DY2906, R&D system, Minneapolis, MN, USA). All groups were analyzed in duplicate.
Human granzyme b elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human Granzyme B ELISA Kit is a sandwich enzyme-linked immunosorbent assay designed for the quantitative measurement of human granzyme B in cell culture supernatants, serum, and plasma. The kit utilizes a specific antibody coated on a 96-well plate to capture the granzyme B protein from the samples. A detection antibody is then added, and the resulting color change is measured using a spectrophotometer.
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Lab products found in correlation
2 protocols using human granzyme b elisa kit
1
Cytotoxicity Assay of NK Cells
2
Granzyme B Quantification in CIK-Tumor Co-culture
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The co‐culture method of tumor cells and CIK cells was the same as before. After 24 h of culture, the cell‐free supernatant was harvested, and the level of granzyme B was determined using the Human Granzyme B Elisa kit (Duoset DY2906‐05 and DY008, R&D Systems, Inc., Minneapolis, MN, USA) following the manufacturer's instructions. Absorbance was measured with an ELISA reader (Infinite® 200 PRO, Tecan) at wavelengths of 450 and 570 nm.
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